Source Paper
Running enhances spatial pattern separation in mice
David J. Creer, Carola Romberg, Lisa M. Saksida, Henriette van Praag, Timothy J. Bussey
Proceedings of the National Academy of Sciences • 2010
Spatial Pattern Separation Task
Objective: Measure the ability of mice to discriminate between the locations of two adjacent identical stimuli and assess how voluntary running affects spatial pattern separation ability
This is a Spatial Pattern Separation Task protocol using mouse as the model organism. The procedure involves 3 procedural steps. Extracted from a 2010 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
mouse • C57BL/6 • male • Adult (3 months old) and very aged (22 months old)
Study window
Estimated timing pending
Core workflow
Subject Selection and Grouping • Running Intervention • Spatial Pattern Separation Task
Primary readouts
- Spatial discrimination ability between adjacent identical stimuli
- Correlation between improved spatial pattern separation and increased neurogenesis
- Age-related differences in spatial pattern separation performance
- Response to running intervention in different age groups
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Subject Selection and Grouping
Adult male C57BL/6 mice aged 3 months and very aged mice aged 22 months were used in the study
Note: Two distinct age groups were tested to compare spatial pattern separation abilities
View evidence from paper
“voluntary running enhanced the ability of adult (3 months old) male C57BL/6 mice to discriminate between the locations of two adjacent identical stimuli”
Running Intervention
Mice were provided with voluntary running access as an intervention condition
Note: Running was voluntary, not forced exercise
View evidence from paper
“Improved spatial pattern separation in adult runners was tightly correlated with increased neurogenesis”
Spatial Pattern Separation Task
Mice were tested on their ability to discriminate between the locations of two adjacent identical stimuli
Note: This task measures fine spatial distinctions mediated by the dentate gyrus
View evidence from paper
“the ability of adult (3 months old) male C57BL/6 mice to discriminate between the locations of two adjacent identical stimuli”