Source Paper
Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age
Hirotaka Shoji, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa
Molecular Brain • 2016
Startle Response and Prepulse Inhibition Test
Objective: Measurement of acoustic startle response and sensorimotor gating through prepulse inhibition
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Equipment1
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Materials4
Software1
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Protocol Steps
Animal transport and acclimation
Genetically engineered mice and wild-type control mice were transported from animal facilities or vendors to the laboratory
Note: Mice were housed in plastic cages with sterilized bedding under 12-hour light/dark cycle with lights on at 7:00 am
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“Genetically engineered mice and their wild-type control mice were transported from the animal facilities of other laboratories or vendors to our laboratory”
Housing conditions
Mice were housed in plastic cages with sterilized PaperClean Bedding under controlled light/dark cycle with ad libitum access to food and water
Note: Housing density varied: 1.3% with 1 animal, 2.3% with 2 animals, 6.7% with 3 animals, 80.1% with 4 animals, 8.9% with 5 animals, and 0.7% with 6 animals per cage
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“housed in plastic cages with sterilized PaperClean Bedding (Japan SLC) under a 12-hr light/dark cycle (lights on at 7:00 am) with access to food (CRF-1, Oriental Yeast Co., Ltd.) and water ad libitum”
Test scheduling
Startle response/prepulse inhibition test was performed as part of a behavioral test battery in a specified order
Note: Startle response/prepulse inhibition test was performed after rotarod and before Porsolt forced swim test. Interval between tests was at least 1 day
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“Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. The mice were generally tested in the following order; general health and neurological screening, light/dark transition, open field, elevated plus maze, hot plate, social interaction, rotarod, startle response/prepulse inhibition, Porsolt forced swim”
Apparatus cleaning and disinfection
All apparatus were cleaned between tests to prevent bias due to olfactory cues
Note: Cleaning performed with super hypochlorous water and 70% ethanol
View evidence from paper
“After the tests, all apparatus were cleaned with super hypochlorous water and 70% ethanol to prevent a bias due to olfactory cues”