Stool Collection Test
Objective: Analyze gastrointestinal function in mice through monthly stool collection during treatment with various interventions
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
Software1
GraphPad Software, Inc., La Jolla, CA, USA • Not mentioned
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Protocol Steps
Animal grouping and treatment assignment
Divide mice into 8 groups with n=10 per group, assigning 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy, rotenone treated after partial sympathectomy) across two treatment time points (2 and 4 months)
Note: Total of 80 mice used across all groups
View evidence from paper
“mice were divided in 8 groups (n = 10) with 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy and rotenone treated after partial sympathectomy) and two treatment times (2 and 4 months)”
Monthly stool collection test
Perform 1-hour stool collection test to analyze gastrointestinal function
Note: Test performed once per month during treatment period
View evidence from paper
“Motor and gastrointestinal functions were analyzed once a month with the help of the rotarod and 1-hour stool collection tests during treatment”
Post-treatment tissue collection
After treatment completion, sacrifice animals and perfuse with 4% paraformaldehyde (PFA) for immunohistological staining and image analysis
Note: Perfusion with 4% PFA performed after treatment period concludes
View evidence from paper
“After treatment, animals were sacrificed and perfused with 4% PFA for immunohistological staining and image analysis”