Deficiency in the Inhibitory Serine-Phosphorylation of Glycogen Synthase Kinase-3 Increases Sensitivity to Mood Disturbances

Abigail Polter, Eléonore Beurel, Sufen Yang, Rakesha Garner, Ling Song et al.

Neuropsychopharmacology • 2010

DOI PMC

Stress-Induced Depressive-Like Behavior Test

behavioralmouseGSK3 α/β 21A/21A/9A/9A knockin mice with serine-to-alanine mutations

Objective: Assessment of stress-induced depressive-like behaviors in GSK3 knockin mice to evaluate mood-related responses and vulnerability to mood disturbances

Protocol Steps

Stress-Induced Depressive-Like Behavior Assessment

Measure behavioral characteristics of GSK3 knockin mice in response to stress to assess depressive-like behaviors

Not specifiedNot specified

Note: GSK3 knockin mice displayed increased susceptibility to stress-induced depressive-like behaviors compared to controls

View evidence from paper

“GSK3 knockin mice displayed increased susceptibility to amphetamine-induced hyperactivity and to stress-induced depressive-like behaviors”

Serine-Phosphorylation Analysis

Measure serine-phosphorylation of GSK3 during mood-related behavioral responses in wild-type mouse brain and blood cells

Not specifiedNot specified

Note: Serine-phosphorylation of GSK3 was reduced during both mood-related behavioral responses

View evidence from paper

“serine-phosphorylation of GSK3 was reduced during both mood-related behavioral responses in wild-type mouse brain and in blood cells from patients with bipolar disorder”