Stress-Induced Depressive-Like Behavior Test
Objective: Assessment of stress-induced depressive-like behaviors in GSK3 knockin mice to evaluate mood-related responses and vulnerability to mood disturbances
This is a Stress-Induced Depressive-Like Behavior Test protocol using mouse as the model organism. The procedure involves 2 procedural steps. Extracted from a 2010 paper published in Neuropsychopharmacology.
Model and subjects
mouse • GSK3 α/β 21A/21A/9A/9A knockin mice with serine-to-alanine mutations • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Stress-Induced Depressive-Like Behavior Assessment • Serine-Phosphorylation Analysis
Primary readouts
- Stress-induced depressive-like behaviors
- Susceptibility to mood disturbances
- Serine-phosphorylation of GSK3 levels
- Behavioral characteristics in response to stress
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Stress-Induced Depressive-Like Behavior Assessment
Measure behavioral characteristics of GSK3 knockin mice in response to stress to assess depressive-like behaviors
Note: GSK3 knockin mice displayed increased susceptibility to stress-induced depressive-like behaviors compared to controls
View evidence from paper
“GSK3 knockin mice displayed increased susceptibility to amphetamine-induced hyperactivity and to stress-induced depressive-like behaviors”
Serine-Phosphorylation Analysis
Measure serine-phosphorylation of GSK3 during mood-related behavioral responses in wild-type mouse brain and blood cells
Note: Serine-phosphorylation of GSK3 was reduced during both mood-related behavioral responses
View evidence from paper
“serine-phosphorylation of GSK3 was reduced during both mood-related behavioral responses in wild-type mouse brain and in blood cells from patients with bipolar disorder”