Administration of CD34+ cells after stroke enhances neurogenesis via angiogenesisin a mouse model

Akihiko Taguchi, Toshihiro Soma, Hidekazu Tanaka, Takayoshi Kanda, Hiroyuki Nishimura et al.

Journal of Clinical Investigation • 2004

DOI PMC

Stroke Model with CD34+ Cell Administration

behavioralmouseimmunocompromised

Objective: Assessment of neurogenesis and functional recovery in immunocompromised mice subjected to stroke and treated with systemic administration of human cord blood-derived CD34+ cells 48 hours post-stroke

Materials & Equipment Checklist

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Materials1

Human cord blood-derived CD34+ cells

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Protocol Steps

Induce stroke in mice

Subject immunocompromised mice to stroke induction procedure

Note: Baseline timepoint for subsequent CD34+ cell administration

View evidence from paper

“immunocompromised mice subjected to stroke”

Administer CD34+ cells post-stroke

Systemic administration of human cord blood-derived CD34+ cells to immunocompromised mice

48 hours post-stroke

Note: Cells administered 48 hours after stroke induction

View evidence from paper

“systemic administration of human cord blood–derived CD34 + cells to immunocompromised mice subjected to stroke 48 hours earlier”

Assess neovascularization

Evaluate neovascularization in the ischemic zone following CD34+ cell administration

Note: Neovascularization is induced in the ischemic zone as a result of CD34+ cell treatment

View evidence from paper

“systemic administration of human cord blood–derived CD34 + cells to immunocompromised mice subjected to stroke 48 hours earlier induces neovascularization in the ischemic zone”

Measure endogenous neurogenesis

Assess endogenous neurogenesis and migration of neuronal progenitor cells to damaged area

Note: Endogenous neurogenesis is accelerated as a result of enhanced migration of neuronal progenitor cells to the damaged area

View evidence from paper

“Endogenous neurogenesis, suppressed by an antiangiogenic agent, is accelerated as a result of enhanced migration of neuronal progenitor cells to the damaged area”

Evaluate neuronal maturation and functional recovery

Assess maturation of neuronal progenitor cells and functional recovery in treated mice

Note: Neuronal progenitor cells mature following migration to damaged area, resulting in functional recovery

View evidence from paper

“followed by their maturation and functional recovery”