Stroke Model with CD34+ Cell Administration
Objective: Assessment of neurogenesis and functional recovery in immunocompromised mice subjected to stroke and treated with systemic administration of human cord blood-derived CD34+ cells 48 hours post-stroke
This is a Stroke Model with CD34+ Cell Administration protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 materials. Extracted from a 2004 paper published in Journal of Clinical Investigation.
Model and subjects
mouse • immunocompromised
Study window
~2 day study window | ~48 hours hands-on
Core workflow
Induce stroke in mice • Administer CD34+ cells post-stroke • Assess neovascularization
Primary readouts
- Neovascularization in the ischemic zone
- Endogenous neurogenesis
- Migration of neuronal progenitor cells to damaged area
- Neuronal maturation
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Induce stroke in mice
Subject immunocompromised mice to stroke induction procedure
Note: Baseline timepoint for subsequent CD34+ cell administration
View evidence from paper
“immunocompromised mice subjected to stroke”
Administer CD34+ cells post-stroke
Systemic administration of human cord blood-derived CD34+ cells to immunocompromised mice
Note: Cells administered 48 hours after stroke induction
View evidence from paper
“systemic administration of human cord blood–derived CD34 + cells to immunocompromised mice subjected to stroke 48 hours earlier”
Assess neovascularization
Evaluate neovascularization in the ischemic zone following CD34+ cell administration
Note: Neovascularization is induced in the ischemic zone as a result of CD34+ cell treatment
View evidence from paper
“systemic administration of human cord blood–derived CD34 + cells to immunocompromised mice subjected to stroke 48 hours earlier induces neovascularization in the ischemic zone”
Measure endogenous neurogenesis
Assess endogenous neurogenesis and migration of neuronal progenitor cells to damaged area
Note: Endogenous neurogenesis is accelerated as a result of enhanced migration of neuronal progenitor cells to the damaged area
View evidence from paper
“Endogenous neurogenesis, suppressed by an antiangiogenic agent, is accelerated as a result of enhanced migration of neuronal progenitor cells to the damaged area”
Evaluate neuronal maturation and functional recovery
Assess maturation of neuronal progenitor cells and functional recovery in treated mice
Note: Neuronal progenitor cells mature following migration to damaged area, resulting in functional recovery
View evidence from paper
“followed by their maturation and functional recovery”