Source Paper
Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice
Dong W, Liu D, Fu S, Zhang J, Chen X et al.
Adv Sci (Weinh) • 2025
Subthreshold Social Defeat Stress
Objective: To induce mild stress that does not cause depression-like behavior on its own but can be used to test stress susceptibility when combined with other manipulations
This is a Subthreshold Social Defeat Stress protocol using Mouse as the model organism. The procedure involves 2 procedural steps. Extracted from a 2025 paper published in Adv Sci (Weinh).
Model and subjects
Mouse • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Subthreshold Social Defeat Stress Administration • Behavioral Testing
Primary readouts
- Social interaction ratio
- Time in interaction zone
- Sucrose preference rate
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Subthreshold Social Defeat Stress Administration
Administer subthreshold social defeat stress to mice
View evidence from paper
“subjected them to subthreshold social defeat stress (SSDS)”
Behavioral Testing
Conduct behavioral assessments following SSDS treatment
View evidence from paper
“subjected them to subthreshold social defeat stress (SSDS), and subsequently performed behavioral tests”