Source Paper
Lipopolysaccharide induces delayed FosB/DeltaFosB immunostaining within the mouse extended amygdala, hippocampus and hypothalamus, that parallel the expression of depressive-like behavior
François Frenois, Maïté Moreau, Jason O’Connor, Marc Lawson, Charlotte Micon et al.
Psychoneuroendocrinology • 2007
Sucrose Consumption Test
Objective: Measurement of sucrose consumption in mice at 24 and 48 hours post-LPS administration as an indicator of anhedonia and depressive-like behavior
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
LPS Administration
Administer lipopolysaccharide to mice via intraperitoneal injection
Note: Dose: 830 μg/kg intraperitoneal
View evidence from paper
“lipopolysaccharide (LPS, 830 μg/kg, intraperitoneal)”
Sucrose Consumption Test at 24 Hours
Measure sucrose consumption in mice at 24 hours post-LPS injection
Note: Test conducted when sickness is expected to be minimal and not bias measurement of depressive-like behavior
View evidence from paper
“LPS also decreased sucrose consumption at 24 and 48 h, despite normal food and water consumption by that time”
Sucrose Consumption Test at 48 Hours
Measure sucrose consumption in mice at 48 hours post-LPS injection
Note: Second measurement timepoint for sucrose consumption
View evidence from paper
“LPS also decreased sucrose consumption at 24 and 48 h, despite normal food and water consumption by that time”