Source Paper
Lipopolysaccharide induces delayed FosB/DeltaFosB immunostaining within the mouse extended amygdala, hippocampus and hypothalamus, that parallel the expression of depressive-like behavior
François Frenois, Maïté Moreau, Jason O’Connor, Marc Lawson, Charlotte Micon et al.
Psychoneuroendocrinology • 2007
Sucrose Consumption Test
Objective: Measurement of sucrose consumption in mice at 24 and 48 hours post-LPS administration as an indicator of anhedonia and depressive-like behavior
This is a Sucrose Consumption Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 4 materials. Extracted from a 2007 paper published in Psychoneuroendocrinology.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
~144 hours hands-on
Core workflow
LPS Administration • Sucrose Consumption Test at 24 Hours • Sucrose Consumption Test at 48 Hours
Primary readouts
- Sucrose consumption at 24 hours post-LPS
- Sucrose consumption at 48 hours post-LPS
- Food consumption
- Water consumption
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
LPS Administration
Administer lipopolysaccharide to mice via intraperitoneal injection
Note: Dose: 830 µg/kg intraperitoneal
View evidence from paper
“lipopolysaccharide (LPS, 830 µg/kg, intraperitoneal)”
Sucrose Consumption Test at 24 Hours
Measure sucrose consumption in mice at 24 hours post-LPS injection
Note: Test conducted when sickness is expected to be minimal and not bias measurement of depressive-like behavior
View evidence from paper
“LPS also decreased sucrose consumption at 24 and 48 h, despite normal food and water consumption by that time”
Sucrose Consumption Test at 48 Hours
Measure sucrose consumption in mice at 48 hours post-LPS injection
Note: Second measurement timepoint for sucrose consumption
View evidence from paper
“LPS also decreased sucrose consumption at 24 and 48 h, despite normal food and water consumption by that time”