Sucrose Preference Test
Objective: Measurement of depressive-like behavior by assessing decreased sucrose preference in mice 24 hours after LPS administration
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Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Ketamine administration
Administer ketamine at 6 mg/kg intraperitoneally immediately before LPS administration
Note: Low dose ketamine used as NMDA receptor antagonist
View evidence from paper
“a low dose of ketamine (6 mg/kg, intraperitoneally) immediately before administration of LPS”
LPS administration
Administer LPS at 0.83 mg/kg intraperitoneally to induce depressive-like behavior
Note: Administered immediately after ketamine in primary protocol
View evidence from paper
“administration of LPS (0.83 mg/kg, intraperitoneally) in C57Bl/6 J mice”
Sucrose preference test
Measure sucrose preference 24 hours after LPS administration as indicator of depressive-like behavior
Note: Decreased sucrose preference indicates depressive-like behavior
View evidence from paper
“Depressive-like behavior was measured 24 h after LPS by decreased sucrose preference”
Forced Swim Test
Measure immobility in forced swim test 24 hours after LPS administration
Note: Increased immobility indicates depressive-like behavior
View evidence from paper
“increased immobility in the forced swim test (FST)”
Alternative ketamine timing - 10 hours post-LPS
In alternative protocol, administer ketamine 10 hours after LPS instead of immediately before
Note: Ketamine still abrogates LPS-induced depressive-like behavior at this timepoint
View evidence from paper
“ketamine was administered 10 h after LPS instead of immediately before LPS”
NBQX pretreatment protocol
Administer NBQX at 10 mg/kg intraperitoneally 15 minutes before ketamine in mice treated with LPS 24 hours earlier
Note: NBQX blocks AMPA receptor enhancement and restores LPS-induced decreased sucrose preference
View evidence from paper
“NBQX administered at the dose of 10 mg/kg intraperitoneally 15 min before ketamine in mice treated with LPS 24 h earlier”