Sucrose Preference Test
Objective: Measurement of depressive-like behavior by assessing decreased sucrose preference in mice 24 hours after LPS administration
This is a Sucrose Preference Test protocol using mouse as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 4 materials. Extracted from a 2013 paper published in Neuropsychopharmacology.
Model and subjects
mouse • C57BL/6J • unknown • Not specified • Not specified
Study window
~1 day study window | ~82.5 hours hands-on
Core workflow
Ketamine administration • LPS administration • Sucrose preference test
Primary readouts
- Sucrose preference (decreased preference indicates depressive-like behavior)
- Immobility in forced swim test (increased immobility indicates depressive-like behavior)
- Body weight loss (sickness measure)
- Motor activity (sickness measure)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Ketamine administration
Administer ketamine at 6 mg/kg intraperitoneally immediately before LPS administration
Note: Low dose ketamine used as NMDA receptor antagonist
View evidence from paper
“a low dose of ketamine (6 mg/kg, intraperitoneally) immediately before administration of LPS”
LPS administration
Administer LPS at 0.83 mg/kg intraperitoneally to induce depressive-like behavior
Note: Administered immediately after ketamine in primary protocol
View evidence from paper
“administration of LPS (0.83 mg/kg, intraperitoneally) in C57Bl/6 J mice”
Sucrose preference test
Measure sucrose preference 24 hours after LPS administration as indicator of depressive-like behavior
Note: Decreased sucrose preference indicates depressive-like behavior
View evidence from paper
“Depressive-like behavior was measured 24 h after LPS by decreased sucrose preference”
Forced Swim Test
Measure immobility in forced swim test 24 hours after LPS administration
Note: Increased immobility indicates depressive-like behavior
View evidence from paper
“increased immobility in the forced swim test (FST)”
Alternative ketamine timing - 10 hours post-LPS
In alternative protocol, administer ketamine 10 hours after LPS instead of immediately before
Note: Ketamine still abrogates LPS-induced depressive-like behavior at this timepoint
View evidence from paper
“ketamine was administered 10 h after LPS instead of immediately before LPS”
NBQX pretreatment protocol
Administer NBQX at 10 mg/kg intraperitoneally 15 minutes before ketamine in mice treated with LPS 24 hours earlier
Note: NBQX blocks AMPA receptor enhancement and restores LPS-induced decreased sucrose preference
View evidence from paper
“NBQX administered at the dose of 10 mg/kg intraperitoneally 15 min before ketamine in mice treated with LPS 24 h earlier”