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Sucrose Preference Test — Jason S. Snyder | ReplicateScience

Experiments/Sucrose Preference Test

Source Paper

Adult hippocampal neurogenesis buffers stress responses and depressive behaviour

Jason S. Snyder, Amélie Soumier, Michelle Brewer, James Pickel, Heather A. Cameron

Nature • 2011

Sucrose Preference Test

behavioralmouseC57Bl/6:CD-1 mixed background, with transgenic v-WT and v-TK mice, and C57Bl/6 mice

Objective: Behavioral test measuring anhedonia-like responses by assessing preference for sucrose solution over water

Materials & Equipment Checklist

8 items

Gather these items before starting the experiment. Check off items as you prepare.

Equipment1

Radioimmunoassay equipment

MP Biomedicals • Not specified • Not specified • Not mentioned

Materials6

Valganciclovir

Not specified • Not specified • Not specified • Not mentioned

Dexamethasone

Sigma • Not specified • Not specified • Not mentioned

Propylene glycol

Not specified • Not specified • Not specified • Not mentioned

BrdU (Bromodeoxyuridine)

Not specified • Not specified • Not specified • Not mentioned

Pentobarbital

Not specified • Not specified • Not specified • Not mentioned

Isoflurane

Not specified • Not specified • Not specified • Not mentioned

Software1

Not specified

Not specified • Not mentioned

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Protocol Steps

1

Animal treatment initiation

Male v-WT and v-TK transgenic mice begin valganciclovir treatment at 8 weeks of age

12 weeks for behavioral experimentsNot specified

Note: Treatment duration varies by experiment type: 8 weeks for dexamethasone, 10-19 weeks for endocrine, 12 weeks for behavior

View evidence from paper

“Male v-WT and v-TK mice were treated with valganciclovir for 8 weeks (dexamethasone experiment), 10-19 weeks (endocrine), 12 weeks (behavior) or 4 weeks (histology; histology after 12 weeks in Supplementary Fig. 1 ), beginning at 8 weeks of age”

2

Behavioral testing

Sucrose preference test performed on mice following treatment completion

Not specifiedNot specified

Note: Different cohorts of mice were tested in different behavioral tests. Sucrose preference test was performed as previously described in referenced publications

View evidence from paper

“Different cohorts of mice were tested in the NSF test, elevated plus maze, forced swim test and sucrose preference test as previously described”

3

Behavioral test timing

Behavioral tests performed either following 30 min of restraint or directly from home cage

30 minutes restraint prior to testing (optional)Not specified

Note: Testing conditions varied between restraint-stressed and home cage conditions

View evidence from paper

“Behavioral tests were performed following 30 min of restraint or directly from the home cage”

Subjects / Specimens

Species: mouse
Strain: C57Bl/6:CD-1 mixed background, with transgenic v-WT and v-TK mice, and C57Bl/6 mice
Age: 8 weeks at start of treatment; tested at various timepoints after treatment
Sex: male
Count: Not specified
Weight: Not specified

Different cohorts of mice were tested in sucrose preference test as previously described

Protocol Summary

Procedure Steps: 3
Materials: 6
Equipment: 1
Outcome Measures: 2