Sucrose Preference Test
Objective: Assessment of anhedonic behaviors through measurement of sucrose preference in mice receiving continuous cerebrospinal fluid infusion
Gather these items before starting the experiment. Check off items as you prepare.
Equipment3
Materials1
Not specified • Not specified • Not specified • Not specified
Software1
Not specified • Not specified
1 item available from ConductScience
Estimated: $2,890.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Surgical implantation of ventricular catheters and osmotic pumps
C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps for continuous cerebrospinal fluid delivery
Note: Catheters connected to osmotic pumps delivering either patients' or control cerebrospinal fluid
View evidence from paper
“C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps that delivered a continuous infusion of patients' or control cerebrospinal fluid”
Continuous cerebrospinal fluid infusion
Osmotic pumps deliver continuous infusion of cerebrospinal fluid to mouse brain
Note: Flow rate maintained at 0.25 µl/h throughout infusion period
View evidence from paper
“osmotic pumps that delivered a continuous infusion of patients' or control cerebrospinal fluid (flow rate 0.25 µl/h, 14 days)”
Sucrose preference test
Standardized behavioral test to assess anhedonic behaviors during and after cerebrospinal fluid infusion period
Note: Test applied during infusion period and after infusion stopped. Measures preference for sucrose solution as indicator of anhedonia
View evidence from paper
“During and after the infusion period standardized tests were applied, including tasks to assess memory (novel object recognition in open field and V-maze paradigms), anhedonic behaviours (sucrose preference test)”
Animal sacrifice and tissue collection
Mice sacrificed at specified timepoints for brain tissue analysis
Note: Sacrifice timepoints: Days 5, 13, 18, 26, and 46 post-infusion initiation
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters”
Brain tissue analysis
Examination of brain tissue for brain-bound antibodies and NMDAR effects using confocal microscopy and immunoblot analysis
Note: Analysis focused on hippocampus; examined total and synaptic NMDAR clusters and protein concentration
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters and protein concentration using confocal microscopy and immunoblot analysis”