Sucrose Preference Test
Objective: Measure anhedonia by assessing preference for sucrose solution over water in a chronic social defeat stress depression model
This is a Sucrose Preference Test protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 6 materials. Extracted from a 2022 paper published in Journal of Neuroinflammation.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
~30 minute study window | ~1 hours hands-on
Core workflow
Chronic Social Defeat Stress (CSDS) Model Implementation • Sucrose Preference Test • Inhibitor Administration
Primary readouts
- Sucrose preference (ratio of sucrose solution consumed to total fluid consumed)
- Anhedonia indicators
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Chronic Social Defeat Stress (CSDS) Model Implementation
Establish depression model through chronic social defeat stress protocol
Note: This is the baseline condition for all subsequent behavioral tests
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Sucrose Preference Test
Conduct sucrose preference test to measure anhedonia by assessing preference for sucrose solution over water
Note: Part of behavioral test battery examining depressive behaviors
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT)”
Inhibitor Administration
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection daily
Note: Intraperitoneal injection route; daily administration schedule
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
EDA Treatment Administration
Administer EDA injection daily following inhibitor injection
Note: Administered 30 minutes after inhibitor injection
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Gpx4 Knockdown via AAV Vector
Perform knockdown experiments using adeno-associated virus vector containing miRNAi (Gpx4)–EGFP infusion to determine effects of Gpx4 on CSDS mice with EDA treatment
Note: Genetic manipulation approach to assess Gpx4 contribution to treatment effects
View evidence from paper
“Knockdown experiments were performed to determine the effects of Gpx4 on CSDS mice with EDA treatment by an adeno-associated virus (AAV) vector containing miRNAi (Gpx4)–EGFP infusion”