Survival Analysis
Objective: Measurement of survival duration in transgenic mice treated with coenzyme Q10 and/or remacemide to evaluate therapeutic effects in Huntington's disease models
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Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Animal treatment initiation
Administer coenzyme Q10 and/or remacemide orally to transgenic mice
Note: Treatment groups included: coenzyme Q10 alone, remacemide alone, and combined coenzyme Q10 and remacemide
View evidence from paper
“oral administration of either coenzyme Q10 or remacemide significantly extended survival and delayed the development of motor deficits”
Monitor survival duration
Track and record survival time in treated R6/2 and N171-82Q transgenic mice
Note: Combined treatment resulted in approximately 32% increase in survival in R6/2 mice and 17% increase in N171-82Q mice
View evidence from paper
“combined treatment, using coenzyme Q10 and remacemide together, was more efficacious than either compound alone, resulting in a ~32 and 17% increase in survival in the R6/2 and N171-82Q mice, respectively”
Monitor motor deficits and weight loss
Assess development of motor deficits and weight loss as secondary outcome measures
Note: Treatment delayed development of these symptoms
View evidence from paper
“delayed the development of motor deficits, weight loss, cerebral atrophy, and neuronal intranuclear inclusions”
Assess cerebral atrophy and neuronal inclusions
Evaluate cerebral atrophy and neuronal intranuclear inclusions in treated mice
Note: Treatment delayed development of these pathological features
View evidence from paper
“delayed the development of motor deficits, weight loss, cerebral atrophy, and neuronal intranuclear inclusions”
Perform magnetic resonance imaging
Conduct MRI scans to measure ventricular enlargement in vivo
Note: Combined treatment significantly attenuated ventricular enlargement
View evidence from paper
“Magnetic resonance imaging showed that combined treatment significantly attenuated ventricular enlargement in vivo”