Objective: Long-term survival monitoring and behavioral characterization of mice across multiple age cohorts (young, mid-age, and old)
This is a Survival and Behavioral Tests protocol using mouse as the model organism. The procedure involves 8 procedural steps, 3 equipment items, 4 materials. Extracted from a 2018 paper published in Nature Communications.
Model and subjects
mouse • Prf1 −/− mice and C57BL/6 (WT controls) • both • 2 months (young), 12 months (mid-age), 24 months (old) • not specified • 5
Study window
Estimated timing pending
Core workflow
Establish mouse cohorts • Monitor mice during first year • Conduct in vivo phenotyping
Primary readouts
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Establish several cohorts of 5 or more mice of both genotypes (Prf1 −/− and C57BL/6 WT controls) for survival and behavioral tests. New cohorts entered the experiment until reaching designated overall cohort size based on previous studies.
Note: Mice assigned to experimental groups according to genotype without randomization or blinding. Equal number of mice from both genders included.
“Several cohorts of 5 or more mice of both genotypes for survival and behavioral tests were established over time and observed for the length of the experiment.”
Observe mice throughout the study period. Exclude any mice that become obese during the first year of experiments from further study.
Note: Mice maintained under specific pathogen-free environment throughout the study
“The mice that become obese during the first year of the experiments were excluded from further study.”
Perform in vivo phenotyping on mice at designated timepoints.
Note: Specific phenotyping methods not detailed in this section
“After in vivo phenotyping, mice were sacrificed at the age of 2 months”
Sacrifice mice at three age timepoints: 2 months (young), 12 months (mid-age), or 24 months (old).
Note: Specific sacrifice method not detailed
“mice were sacrificed at the age of 2 months ('young'), 12 months ('mid age'), or 24 months ('old')”
Collect heparinized blood and plasma from sacrificed mice.
Note: Blood count and biochemistry laboratory was blind to sample identity
“Heparinized blood and plasma were collected. The blood count and biochemistry laboratory was blind to the sample identity.”
Prepare single-cell suspensions from perfused liver, pancreas, lungs, and skin for analysis by flow cytometry and ImageStreamX analysis.
Note: Tissues must be perfused before suspension preparation
“Single-cell suspensions from perfused liver, pancreas, lungs, and skin were taken for analysis by flow cytometry and ImageStream®X analysis.”
Fix tissues in formalin and embed in paraffin blocks for histological analysis.
Note: Alternative to flow cytometry analysis
“tissues were fixed in formalin and embedded in paraffin blocks”
Immediately embed tissues in OCT and store at −80 °C for frozen section analysis.
Note: Alternative to formalin fixation
“tissues were immediately embedded in OCT and kept at −80 °C”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Long-term survival monitoring and behavioral characterization of mice across multiple age cohorts (young, mid-age, and old)
Objective
Long-term survival monitoring and behavioral characterization of mice across multiple age cohorts (young, mid-age, and old)
Subjects
From papermouse • Prf1 −/− mice and C57BL/6 (WT controls) • both • 2 months (young), 12 months (mid-age), 24 months (old) • not specified
Sample count
From paper5
Cohort notes
From paperEqual number of mice from both genders included; mice that became obese during first year were excluded; assignment to experimental groups by genotype without randomization or blinding
Establish mouse cohorts (not specified)
Monitor mice during first year (12 months)
Conduct in vivo phenotyping (not specified)
Sacrifice mice at designated ages (not specified)
Survival duration across age cohorts
From paperBlood count and biochemistry laboratory analysis was conducted blind to sample identity
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Behavioral characteristics at different ages
From paperBlood count and biochemistry laboratory analysis was conducted blind to sample identity
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Blood count parameters
From paperBlood count and biochemistry laboratory analysis was conducted blind to sample identity
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Biochemistry parameters
From paperBlood count and biochemistry laboratory analysis was conducted blind to sample identity
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Survival duration across age cohorts
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Behavioral characteristics at different ages
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Blood count parameters
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Biochemistry parameters
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
Blood count and biochemistry laboratory analysis was conducted blind to sample identity
Scoring or quantification
Quantify the primary readouts for this experiment: Survival duration across age cohorts; Behavioral characteristics at different ages; Blood count parameters; Biochemistry parameters.
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Survival duration across age cohorts, Behavioral characteristics at different ages, Blood count parameters, Biochemistry parameters.
Source links and direct wording from the methods section for validation and deeper review.
Citation
Yossi Ovadya et al. (2018). Impaired immune surveillance accelerates accumulation of senescent cells and aging. Nature Communications
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Current status surfaces were computed from experiment data updated Feb 28, 2026.
Source access
Jump back into the original paper or the methods evidence section when you need exact wording, exclusions, or method-specific caveats.
This protocol has structured steps plus evidence quotes, and is ready for canonical sync.
Steps
8
Evidence Quotes
15
Protocol Items
7
Linked Products
0
Canonical Sync
Pending
What this means
The completeness score reflects how much structured protocol data is present: steps, methods evidence, listed materials, linked products, and paper provenance.
Computed from the current experiment record updated Feb 28, 2026.
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Steps
8
Evidence
15
Specific Products
0/0
Canonical Sync
Pending
What this score means
The verification score reflects evidence coverage, subject detail, paper provenance, step depth, and whether linked products resolve to specific item pages instead of generic searches.
Computed from the current experiment record updated Feb 28, 2026.
A page can have structured steps and still need review when evidence is thin, product links are generic, or canonical protocol coverage is still pending.
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