Source Paper
Disruption of the Metallothionein-III Gene in Mice: Analysis of Brain Zinc, Behavior, and Neuron Vulnerability to Metals, Aging, and Seizures
Jay C. Erickson, Gunther Hollopeter, Steven A. Thomas, Glenda J. Froelick, Richard D. Palmiter
Journal of Neuroscience • 1997
Systemic Metal Exposure Testing
Objective: Assessment of sensitivity to systemic zinc or cadmium exposure in MT-III-deficient and wild-type mice
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Equipment1
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Protocol Steps
Systemic metal exposure
Mice were exposed systemically to zinc or cadmium to assess sensitivity
Note: MT-III-deficient mice were not sensitive to this exposure, unlike wild-type controls
View evidence from paper
“were not sensitive to systemic zinc or cadmium exposure”
Morris water maze testing
Spatial learning assessment was conducted in the Morris water maze
Note: MT-III-deficient mice exhibited normal spatial learning
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“Mutant mice exhibited normal spatial learning in the Morris water maze”
Kainic acid-induced seizure testing
Mice were administered kainic acid to induce seizures and assess vulnerability
Note: MT-III-deficient mice were more susceptible to seizures; transgenic mice with elevated MT-III were more resistant
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“more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury”
Long-term behavioral and neuropathological assessment
Behavioral deficits and neuropathology were evaluated in 2-year-old MT-III-deficient mice
Note: No neuropathology or behavioral deficits were detected at this age
View evidence from paper
“No neuropathology or behavioral deficits were detected in 2-year-old MT-III-deficient mice”