Source Paper
Disruption of the Metallothionein-III Gene in Mice: Analysis of Brain Zinc, Behavior, and Neuron Vulnerability to Metals, Aging, and Seizures
Jay C. Erickson, Gunther Hollopeter, Steven A. Thomas, Glenda J. Froelick, Richard D. Palmiter
Journal of Neuroscience • 1997
Systemic Metal Exposure Testing
Objective: Assessment of sensitivity to systemic zinc or cadmium exposure in MT-III-deficient and wild-type mice
This is a Systemic Metal Exposure Testing protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 3 materials. Extracted from a 1997 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not explicitly stated • unknown • Not explicitly stated • Not explicitly stated
Study window
Estimated timing pending
Core workflow
Systemic metal exposure • Morris water maze testing • Kainic acid-induced seizure testing
Primary readouts
- Sensitivity to systemic zinc exposure
- Sensitivity to systemic cadmium exposure
- Spatial learning performance in Morris water maze
- Susceptibility to kainic acid-induced seizures
Key equipment and reagents
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Protocol Steps
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Systemic metal exposure
Mice were exposed systemically to zinc or cadmium to assess sensitivity
Note: MT-III-deficient mice were not sensitive to this exposure, unlike wild-type controls
View evidence from paper
“were not sensitive to systemic zinc or cadmium exposure”
Morris water maze testing
Spatial learning assessment was conducted in the Morris water maze
Note: MT-III-deficient mice exhibited normal spatial learning
View evidence from paper
“Mutant mice exhibited normal spatial learning in the Morris water maze”
Kainic acid-induced seizure testing
Mice were administered kainic acid to induce seizures and assess vulnerability
Note: MT-III-deficient mice were more susceptible to seizures; transgenic mice with elevated MT-III were more resistant
View evidence from paper
“more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury”
Long-term behavioral and neuropathological assessment
Behavioral deficits and neuropathology were evaluated in 2-year-old MT-III-deficient mice
Note: No neuropathology or behavioral deficits were detected at this age
View evidence from paper
“No neuropathology or behavioral deficits were detected in 2-year-old MT-III-deficient mice”