Source Paper
Aerobic Exercise Promotes Hippocampal Neurogenesis and Ameliorates Cognitive Dysfunction Induced by Unilateral Labyrinthectomy
Zhou Z, Yu X, Tian E, Guo Z, Chen J et al.
CNS Neurosci Ther • 2026
T-Maze
Objective: Assessment of spatial learning and memory by measuring preference for previously closed arm exploration
This is a T-Maze protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 3 equipment items, 1 materials. Extracted from a 2026 paper published in CNS Neurosci Ther.
Model and subjects
Mouse • C57BL/6J • Male only • 8 weeks old • Not specified
Study window
~40 minutes hands-on
Core workflow
Pre-test acclimatization • Learning phase • Inter-trial interval
Primary readouts
- Percentage of entries into closed arms during acquisition phase
- Total exploration time in closed arms during acquisition phase
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Pre-test acclimatization
Place mice in testing room for acclimatization
View evidence from paper
“mice were placed in the testing room 24 h before the experiment”
Learning phase
Only one arm of the T-maze (either left or right) is open, allowing mouse to freely explore
View evidence from paper
“only one arm of the T‐maze (either the left or the right) was open, allowing the mouse to freely explore the maze for 5 min”
Inter-trial interval
Return mouse to home cage between phases
View evidence from paper
“before returning to its home cage. Following a 30‐min interval”
Testing phase
Both arms of the T-maze are open and mouse is permitted to explore freely
View evidence from paper
“both arms of the T‐maze were open, and the mouse was permitted to explore the maze freely for another 5 min”