Source Paper
Voluntary exercise produces antidepressant and anxiolytic behavioral effects in mice
Catharine H. Duman, Lee Schlesinger, David S. Russell, Ronald S. Duman
Brain Research • 2008
Tail Suspension Test
Objective: Assessment of antidepressant-like behavioral effects in mice following chronic wheel running using the tail suspension test paradigm
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
Materials1
Not specified in provided text • Not specified in provided text • Not specified in provided text • Not specified in provided text
Software1
Not specified in provided text • Not specified in provided text
1 item available from ConductScience
Estimated: $3,899.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Provide voluntary wheel running access
Mice were given free access to running wheels for chronic exercise
Note: This is the intervention condition compared to sedentary control mice
View evidence from paper
“Mice given free access to running wheels showed antidepressant-like behavior”
Conduct tail suspension test
Mice were tested in the tail suspension paradigm to assess antidepressant-like behavioral effects
Note: Test was performed on both exercising and sedentary control mice
View evidence from paper
“tail suspension paradigms”
Measure behavioral responses
Behavioral responses in tail suspension test were recorded and compared between exercising and control mice
Note: Responses were similar to antidepressant drug-treated animals
View evidence from paper
“These responses were similar to responses of antidepressant drug-treated animals”
Test BDNF knockout mice
Heterozygous BDNF knockout mice were tested in tail suspension paradigm after wheel-running
Note: Exercise was ineffective in improving performance in BDNF heterozygous knockout mice
View evidence from paper
“exercise was ineffective in improving FST performance in heterozygous BDNF knockout mice”
Administer MEK inhibitor
Subchronic administration of PD184161 was given to exercising mice to block MAPK signaling pathway
Note: This blocked the antidepressant-like behavioral response in the tail suspension test
View evidence from paper
“Subchronic administration of PD184161 to exercising mice blocked the antidepressant-like behavioral response”