Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Surgical implantation of ventricular catheters and osmotic pumps
C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps for continuous infusion delivery
Note: Catheters and pumps were connected for continuous infusion
View evidence from paper
“C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps that delivered a continuous infusion”
Continuous cerebrospinal fluid infusion
Continuous infusion of either patients' cerebrospinal fluid or control cerebrospinal fluid through the osmotic pumps
Note: Flow rate maintained at 0.25 µl/h throughout infusion period
View evidence from paper
“osmotic pumps that delivered a continuous infusion of patients' or control cerebrospinal fluid (flow rate 0.25 µl/h, 14 days)”
Tail suspension test
Standardized test applied during and after infusion period to assess depressive-like behaviors
Note: Test was performed as part of comprehensive behavioral battery including memory, anhedonia, anxiety, aggression, and locomotor activity assessments
View evidence from paper
“During and after the infusion period standardized tests were applied, including tasks to assess memory (novel object recognition in open field and V-maze paradigms), anhedonic behaviours (sucrose preference test), depressive-like behaviours (tail suspension, forced swimming tests)”
Animal sacrifice and tissue collection
Animals were sacrificed at specified timepoints for examination of brain-bound antibodies and NMDAR effects
Note: Sacrifice occurred at Days 5, 13, 18, 26, and 46 post-infusion initiation
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters”
Confocal microscopy analysis
Examination of brain tissue for brain-bound antibodies and effects on NMDAR clusters using confocal microscopy
Note: Analysis focused on hippocampus where antibodies were predominantly localized
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters and protein concentration using confocal microscopy and immunoblot analysis”
Immunoblot analysis
Analysis of protein concentration and NMDAR levels in brain tissue
Note: Examined total and synaptic NMDAR clusters and protein concentration
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters and protein concentration using confocal microscopy and immunoblot analysis”