Prenatal and early postnatal cannabis exposure interactions with adolescent chronic stress on anxiety-like, depression-like, and risk-taking behaviour.

Peterson CS, Ifionu I, Hamood F, Semizeh H, Ali A et al.

Psychopharmacology (Berl) • 2026

PMC

Tail Suspension Test

behavioralmouse

Objective: Measures stress coping behavior in mice

Protocol Steps

Prenatal and postnatal THC exposure

Mouse dams orally consumed THC in whole cannabis oil daily during gestation and early postnatal period

GD1-PD10

Note: 5 mg/kg THC dose in whole cannabis oil

View evidence from paper

“Mouse dams orally consumed 5 mg/kg THC in whole cannabis oil daily from GD1-PD10”

Chronic mild unpredictable stress exposure

Offspring were exposed to chronic mild unpredictable stress throughout adolescence

PD28-56

Note: Applied during adolescent period

View evidence from paper

“Offspring were exposed to chronic mild unpredictable stress throughout adolescence (PD28-56)”

Behavioral testing battery initiation

Mice were challenged with a battery of behavioral tests including tail suspension test

From PD58

Note: Part of comprehensive behavioral assessment including anxiety-like, stress coping, anhedonia-like, risk-taking, and social motivation tests

View evidence from paper

“From PD58, mice were challenged with a battery of tests to measure anxiety-like (elevated plus maze, open field test), stress coping (forced swim test, tail suspension test)”