Tail Suspension Test
Objective: Measure depression-like behavior by assessing immobility time when a mouse is suspended by the tail
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
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Materials4
Software1
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Protocol Steps
Establish CSDS depression model
Perform chronic social defeat stress (CSDS) to induce depression-like phenotype in mice
Note: This is the baseline condition before behavioral testing
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Administer inhibitors
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection
Note: Inhibitors administered daily
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Administer EDA treatment
Inject EDA daily following inhibitor administration
Note: Administered after 30-minute interval from inhibitor injection
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Perform tail suspension test
Suspend mouse by the tail and measure immobility time as an indicator of depression-like behavior
Note: Part of comprehensive behavioral testing battery
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT), open field test (OFT), elevated plus maze (EPM), novel object recognition (NOR), tail suspension test (TST) and forced swim test (FST)”
Perform AAV-mediated Gpx4 knockdown
Infuse adeno-associated virus vector containing miRNAi (Gpx4)–EGFP to knockdown Gpx4 expression in CSDS mice treated with EDA
Note: Separate experimental group to determine Gpx4 effects
View evidence from paper
“Knockdown experiments were performed to determine the effects of Gpx4 on CSDS mice with EDA treatment by an adeno-associated virus (AAV) vector containing miRNAi (Gpx4)–EGFP infusion”