Source Paper
Computational modeling reveals cognitive processes in simple rodent depression tests
Li Z, Lu T, Yan J, Zhang X, Li YF
Cell Rep Methods • 2025
Tail Suspension Test (TST)
behavioralMouseC57Bl/6
Objective: To assess depressive-like behavior and behavioral despair in mice through immobility measurement during tail suspension
Protocol Steps
1
Remove mice from cages
Gently remove mice from their home cages
View evidence from paper
“mice were gently removed from their cages”
2
Secure tail with tape
Attach adhesive tape to the tail approximately 1 cm from the tip
View evidence from paper
“their tails were secured with adhesive tape approximately 1 cm from the tip”
3
Suspend mice by tail
Suspend the mice by their tails at a height of about 30 cm from the ground in an inverted position
View evidence from paper
“mice were then suspended by their tails at a height of about 30 cm from the ground, placing them in an inverted position”
4
Conduct 6-minute test
Allow the test to run for 6 minutes while recording video throughout the entire duration
View evidence from paper
“The test also lasted for 6 min, and video recordings were taken throughout”