Source Paper
Kappa opioid receptor antagonism and prodynorphin gene disruption block stress-induced behavioral responses.
Jay P McLaughlin, Monica Marton-Popovici, Charles Chavkin
PubMed • 2003
Tail Withdrawal Latency Assay
Objective: Measurement of stress-induced analgesia by assessing tail withdrawal latency in response to heat
This is a Tail Withdrawal Latency Assay protocol using mouse as the model organism. The procedure involves 5 procedural steps, 3 equipment items, 2 materials. Extracted from a 2003 paper published in PubMed.
Model and subjects
mouse • C57Bl/6 • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Forced Swim Testing (FST) • Tail Withdrawal Latency Assessment • nor-BNI Pretreatment (experimental group)
Primary readouts
- Tail withdrawal latency (measure of stress-induced analgesia)
- Stress-induced immobility response during FST
- Cocaine-conditioned place preference magnitude
- Effect of nor-BNI on stress-induced behavioral responses
Key equipment and reagents
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Protocol Steps
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Forced Swim Testing (FST)
Subjects undergo repeated forced swim testing using a modified Porsolt procedure
Note: This is the stress exposure procedure
View evidence from paper
“C57Bl/6 mice subjected to repeated forced swim testing (FST) using a modified Porsolt procedure at 30°C”
Tail Withdrawal Latency Assessment
Measure tail withdrawal latency in response to heat to assess stress-induced analgesia
Note: Performed following FST to measure analgesia response
View evidence from paper
“stress-induced analgesia observed with a tail withdrawal latency assay”
nor-BNI Pretreatment (experimental group)
Administer κ opioid receptor antagonist nor-binaltorphimine via intraperitoneal injection prior to FST
Note: Pretreatment condition to test κ opioid receptor involvement
View evidence from paper
“Pretreatment with the κ opioid receptor antagonist nor-binaltorphimine (nor-BNI; 10 mg/kg, i.p.)”
Cocaine Conditioning
Condition FST-exposed mice with cocaine via subcutaneous injection for place preference testing
Note: Dose: 15 mg/kg, s.c.
View evidence from paper
“FST-exposed mice conditioned with cocaine (15 mg/kg, s.c.)”
Conditioned Place Preference (CPP) Testing
Measure place preference for drug-paired chamber in FST-exposed mice versus unstressed controls
Note: Assess potentiation of cocaine reward by stress
View evidence from paper
“FST-exposed mice conditioned with cocaine (15 mg/kg, s.c.) showed significant potentiation of place preference for the drug-paired chamber”