Source Paper
Cannabidiol attenuates seizures and social deficits in a mouse model of Dravet syndrome
Joshua S. Kaplan, Nephi Stella, William A. Catterall, Ruth E. Westenbroek
Proceedings of the National Academy of Sciences • 2017
Thermally Induced Seizure Assessment
Objective: Measurement of duration and severity of seizures induced by thermal stimulation in Dravet syndrome mice to assess cannabidiol treatment effects
This is a Thermally Induced Seizure Assessment protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items. Extracted from a 2017 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
mouse • Dravet syndrome mouse model (Scn1a mutation) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Thermal seizure induction • Seizure measurement
Primary readouts
- Duration of thermally induced seizures
- Severity of thermally induced seizures
- Frequency of spontaneous seizures
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Thermal seizure induction
Expose Dravet syndrome mice to thermal stimulation to induce seizures
Note: This is the primary experimental manipulation for assessing seizure phenotype
View evidence from paper
“The duration and severity of thermally induced seizures and the frequency of spontaneous seizures were substantially decreased.”
Seizure measurement
Measure and record the duration and severity of induced seizures
Note: Both duration and severity parameters are assessed
View evidence from paper
“The duration and severity of thermally induced seizures and the frequency of spontaneous seizures were substantially decreased.”