Source Paper
Amygdala Inputs to the Ventral Hippocampus Bidirectionally Modulate Social Behavior
Ada C. Felix-Ortiz, Kay M. Tye
Journal of Neuroscience • 2014
Three Chamber Sociability Test
Objective: Measure social interaction and sociability in mice by quantifying time spent in social zones during a three chamber test
This is a Three Chamber Sociability Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 2 materials. Extracted from a 2014 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Viral transduction of BLA pyramidal neurons • Chronic implantation of optical fibers • Three chamber sociability test
Primary readouts
- Time spent in social zone
- Social interaction behavior
- Sociability measures
Key equipment and reagents
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Protocol Steps
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Viral transduction of BLA pyramidal neurons
BLA pyramidal neurons were transduced using adeno-associated viral vectors carrying either ChR2 or NpHR under the control of the CaMKII-alpha promoter
Note: Promoter control allows for optical excitation or inhibition of amygdala axon terminals
View evidence from paper
“BLA pyramidal neurons were transduced using adeno-associated viral vectors (AAV 5) carrying either channelrhodopsin-2 (ChR2) or halorhodopsin (NpHR), under the control of the CaMKII-alpha promoter”
Chronic implantation of optical fibers
Optical fibers were chronically implanted to allow selective manipulation of BLA terminals in the ventral hippocampus
Note: Fibers enable optical control of the BLA-vHPC projection pathway
View evidence from paper
“Optical fibers were chronically implanted to selectively manipulate BLA terminals in the vHPC”
Three chamber sociability test
Mice are placed in a three chamber apparatus and time spent in the social zone is measured
Note: Test measures social interaction and sociability; can be combined with optogenetic manipulation
View evidence from paper
“in the three chamber sociability test by decreased time spent in the social zone”