Three-Chamber Social Interaction Assay
behavioralmouseC57BL/6J
Objective: Assessment of social interaction preference by measuring mouse interaction with a novel object versus a stranger mouse in a three-chamber assay
Protocol Steps
1
Habituation to three-chamber box
Test mice are habituated to the three-chamber box prior to social interaction testing
Note: Duration of habituation not specified in methods
View evidence from paper
“After habituating to the three-chamber box, the test mice were given a choice”
2
Social interaction choice test
Test mice are given a choice of either interacting with a novel object or a stranger mouse
Note: Specific duration of test not stated in methods
View evidence from paper
“the test mice were given a choice of either interacting with a novel object or a stranger mouse”
3
Measurement of interaction preference
Quantify duration and frequency of interaction with stranger mouse versus novel object
Note: Analysis performed by observer blinded to genotype using automated tracking software
View evidence from paper
“We found that while WT mice demonstrated strong preference for the stranger mouse over the novel object, KO mice displayed no such preference”