Two-Choice Swim Tank
Objective: Assess discrimination learning ability in mice using a two-choice swimming paradigm to evaluate cognitive function sensitive to frontostriatal and hippocampal function
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
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Protocol Steps
Subject preparation and grouping
R6/2 transgenic mice and their wild-type littermates were assigned to behavioral testing groups
Note: Separate groups of mice were used for each test to avoid confounding effects of repeated testing
View evidence from paper
“Behavioral testing was performed on R6/2 transgenic mice and their wild-type littermates between 3 and 14.5 weeks of age, using separate groups of mice for each test”
Two-choice swim tank discrimination learning task
Mice performed discrimination learning task in the two-choice swim tank requiring selection between two options during swimming
Note: Task was used to assess cognitive performance and detect learning impairments
View evidence from paper
“two-choice swim tank, and T-maze tasks. Behavioral testing was performed on R6/2 transgenic mice and their wild-type littermates between 3 and 14.5 weeks of age”
Assessment period
Behavioral testing conducted across multiple age timepoints to track progressive changes in cognitive performance
Note: Progressive deterioration in learning was assessed between 3.5 and 8 weeks of age
View evidence from paper
“between 3.5 and 8 weeks of age, R6/2 mice displayed progressive deterioration in specific aspects of learning in the Morris water maze, visual cliff, two-choice swim tank, and T-maze tasks”