Source Paper
Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis
Tang T, Xu J, Fu B, Geng C, Li Y et al.
CNS Neurosci Ther • 2026
Video Electroencephalogram Recording
Objective: Video electroencephalogram recording for 24-hour continuous monitoring of seizure activity and assessment of seizure severity in mouse models
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Protocol Steps
Electrode placement preparation
Position monitoring electrode around hippocampus projection, ground and reference electrodes at specified coordinates
View evidence from paper
“single point monitoring electrode around the skull surface of the hippocampus projection (the central cranial planar projection coordinates: AP‐2.50, ML ±2.50)”
Electrode installation
Insert three stainless steel screws into skull and fix with dental cement
View evidence from paper
“skull electrodes were then inserted and securely fixed using dental cement”
Wire connection
Connect silver wires from screws to row pins for signal transmission
View evidence from paper
“These screws were then entwined by wrapped silver wires connected to the needle of the row pins”
Signal acquisition setup
Connect mice to NeuSen H system starting on 8th day post-surgery
View evidence from paper
“mice were connected to the NeuSen H signal acquisition system for 24‐h continuous monitoring, commencing on the 8th day post‐surgery”
Recording duration
Perform continuous 24-hour monitoring for 3 consecutive weeks
View evidence from paper
“24‐h continuous monitoring, commencing on the 8th day post‐surgery and continuing for the subsequent 3 weeks”
Signal processing
Apply EEG filter settings and determine seizure onset/termination based on EEG signals
View evidence from paper
“EEG filter settings adjusted to 1–70 Hz and the notch filter activated”