Objective: Assessment of influenza virus pathogenesis through intranasal inoculation of mice with varying viral doses, monitoring body weight changes and survival
This is a Viral Pathogenesis in Mice protocol using mouse as the model organism. The procedure involves 7 procedural steps, 2 equipment items, 9 materials. Extracted from a 2009 paper published in Nature.
Model and subjects
mouse • BALB/c • female • Six-week-old • Not specified • 3
Study window
~2 week study window
Core workflow
Baseline body weight measurement • Anesthetize mice • Intranasal inoculation with varying viral doses
Primary readouts
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Measure and record baseline body weights of all mice prior to viral inoculation
Note: Essential for determining 25% weight loss threshold for euthanasia
“Baseline body weights were measured prior to infection”
Anesthetize mice with isoflurane prior to intranasal inoculation
Note: Required for safe intranasal inoculation procedure
“Three mice per group were anesthetized with isoflurane and intranasally inoculated”
Intranasally inoculate anesthetized mice with 50 µl of viral suspension at doses of 10^2, 10^3, 10^4, or 10^5 PFU of CA04 and KUTK-4, or undiluted virus stocks (CA04; 10^6.5 PFU, KUTK-4; 10^6.6 PFU)
Note: Three mice per group for survival monitoring; 6 mice per group for virologic and pathologic examinations
“Three mice per group were anesthetized with isoflurane and intranasally inoculated with 10^2, 10^3, 10^4, or 10^5 PFU (50 µl) of CA04 and KUTK-4, or undiluted virus from virus stocks”
Monitor and record body weight of all mice daily for 14 days post-infection
Note: Mice with body weight loss exceeding 25% of pre-infection values must be euthanized
“Body weight and survival were monitored daily for 14 days and mice with body weight loss of more than 25% of pre-infection values were euthanized”
Monitor and record survival status of all mice daily for 14 days post-infection
Note: Record any deaths and their timing
“Body weight and survival were monitored daily for 14 days”
Euthanize 3 mice per group on days 3 and 6 post-infection for virologic and pathologic examinations
Note: 6 mice per group total were intranasally infected with 10^5 PFU of S-OIVs and KUTK-4
“For virologic and pathologic examinations, 6 mice per group were intranasally infected with 10^5 PFU of S-OIVs and KUTK-4 and 3 mice per group were euthanized on days 3 and 6 pi”
Determine virus titres in various organs from euthanized mice using plaque assays in MDCK cells
Note: Performed on tissue samples from mice euthanized on days 3 and 6 post-infection
“The virus titres in various organs were determined by plaque assays in MDCK cells”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Assessment of influenza virus pathogenesis through intranasal inoculation of mice with varying viral doses, monitoring body weight changes and survival
Objective
Assessment of influenza virus pathogenesis through intranasal inoculation of mice with varying viral doses, monitoring body weight changes and survival
Subjects
From papermouse • BALB/c • female • Six-week-old • Not specified
Sample count
From paper3
Cohort notes
From paperBaseline body weights measured prior to infection
Baseline body weight measurement (Not specified)
Anesthetize mice (Not specified)
Intranasal inoculation with varying viral doses (Not specified)
Daily body weight monitoring (14 days)
Body weight changes (daily monitoring for 14 days)
From paperNot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Survival (daily monitoring for 14 days)
From paperNot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Viral titers in various organs (plaque assay results)
From paperNot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Pathologic findings (from tissue examination)
From paperNot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Body weight changes (daily monitoring for 14 days)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Survival (daily monitoring for 14 days)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Viral titers in various organs (plaque assay results)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Pathologic findings (from tissue examination)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
Not specified in methods section
Scoring or quantification
Quantify the primary readouts for this experiment: Body weight changes (daily monitoring for 14 days); Survival (daily monitoring for 14 days); Viral titers in various organs (plaque assay results); Pathologic findings (from tissue examination).
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Body weight changes (daily monitoring for 14 days), Survival (daily monitoring for 14 days), Viral titers in various organs (plaque assay results), Pathologic findings (from tissue examination).
Source links and direct wording from the methods section for validation and deeper review.
Citation
Yasushi Itoh et al. (2009). In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses. Nature
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Current status surfaces were computed from experiment data updated Feb 28, 2026.
Source access
Jump back into the original paper or the methods evidence section when you need exact wording, exclusions, or method-specific caveats.
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Steps
7
Evidence Quotes
18
Protocol Items
11
Linked Products
0
Canonical Sync
Pending
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Computed from the current experiment record updated Feb 28, 2026.
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Steps
7
Evidence
18
Specific Products
0/0
Canonical Sync
Pending
What this score means
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Computed from the current experiment record updated Feb 28, 2026.
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