Source Paper
Tobias Plümpe, Dan Ehninger, Barbara Steiner, Friederike Klempin, Sebastian Jessberger et al.
BMC Neuroscience • 2006
Objective: To assess effects of voluntary physical activity on neurogenesis by housing mice with running wheels and analyzing dendritic morphology and neurogenesis markers
This is a Voluntary Running Wheel Activity protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 4 materials. Extracted from a 2006 paper published in BMC Neuroscience.
Model and subjects
mouse • C57BL/6 • female • 6 weeks old • 19-24 g • 18
Study window
~6 week study window
Core workflow
Animal grouping and housing setup • Kainic acid injection for seizure group • BrdU injections
Primary readouts
Key equipment and reagents
Verified items
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Divide 18 female C57BL/6 mice (6 weeks old, 19-24g) into 3 groups: seizure (N=6), running (N=6), and controls (N=6). House running group with 2-3 animals per cage equipped with running wheel.
Note: Running group housed with running wheel access
“Additional 18 female C57BL/6 mice (same age as above) were divided into 3 groups: seizure (N = 6), running (N = 6) and controls (N = 6). The Runner group was housed with 2–3 animals per cage that was equipped with a running wheel.”
Administer single intraperitoneal injection of 30 mg/kg kainic acid in 0.1 M PBS to seizure group on Day 0 (day before BrdU injections). Only animals displaying continuous convulsive seizure activity are used in experiments.
Note: Only seizure animals with continuous convulsive seizure activity are included
“Seizure animals received a single intraperitoneal application of 30 mg/kg kainic acid (KA, Sigma) in 0.1 M phosphate buffered saline (PBS) on the day before BrdU-injections (Day 0), and only those displaying continuous convulsive seizure activity were used in these experiments.”
Administer daily intraperitoneal injections of BrdU (50 mg/kg in sterile 0.9% NaCl) to all animals for 7 consecutive days starting on Day 1 (day after kainic acid injection for seizure group).
Note: All three groups (seizure, running, controls) receive BrdU injections
“During the first 7 days of the experiment all animals received one daily injection of BrdU.”
Running group animals have continuous access to running wheels throughout the experimental period while housed 2-3 per cage.
Note: Running wheel access is the key intervention for the running group
“The Runner group was housed with 2–3 animals per cage that was equipped with a running wheel.”
Collect tissue from all groups for analysis of dendritic morphology and neurogenesis markers. Data on dendritic morphology were exclusively generated for the present study.
Note: Focus on dendritic morphology analysis specific to this study
“Data on dendritic morphology, etc., were exclusively generated for the present study.”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
To assess effects of voluntary physical activity on neurogenesis by housing mice with running wheels and analyzing dendritic morphology and neurogenesis markers
Objective
To assess effects of voluntary physical activity on neurogenesis by housing mice with running wheels and analyzing dendritic morphology and neurogenesis markers
Subjects
From papermouse • C57BL/6 • female • 6 weeks old • 19-24 g
Sample count
From paper18
Cohort notes
From paperDivided into 3 groups: seizure (N=6), running (N=6), and controls (N=6)
Animal grouping and housing setup
Kainic acid injection for seizure group
BrdU injections (7 days, once daily)
Voluntary running activity (Throughout 7-day BrdU injection period and beyond)
Dendritic morphology of DCX-positive cells
From paperNot explicitly described in the provided methods text
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Spatial relationship between DCX-positive cells and astrocytes
From paperNot explicitly described in the provided methods text
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Cell proliferation (BrdU incorporation)
From paperNot explicitly described in the provided methods text
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Apoptosis detection
From paperNot explicitly described in the provided methods text
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Dendritic morphology of DCX-positive cells
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Spatial relationship between DCX-positive cells and astrocytes
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Cell proliferation (BrdU incorporation)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Apoptosis detection
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
Not explicitly described in the provided methods text
Scoring or quantification
Quantify the primary readouts for this experiment: Dendritic morphology of DCX-positive cells; Spatial relationship between DCX-positive cells and astrocytes; Cell proliferation (BrdU incorporation); Apoptosis detection.
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Dendritic morphology of DCX-positive cells, Spatial relationship between DCX-positive cells and astrocytes, Cell proliferation (BrdU incorporation), Apoptosis detection.
Source links and direct wording from the methods section for validation and deeper review.
Citation
Tobias Plümpe et al. (2006). Variability of doublecortin-associated dendrite maturation in adult hippocampal neurogenesis is independent of the regulation of precursor cell proliferation. BMC Neuroscience
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5
Evidence Quotes
10
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Evidence
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Canonical Sync
Pending
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