Experiments/Y-Maze Behavioral Test

Source Paper

-Amyloid Oligomers Induce Phosphorylation of Tau and Inactivation of Insulin Receptor Substrate via c-Jun N-Terminal Kinase Signaling: Suppression by Omega-3 Fatty Acids and Curcumin

Q.-L. Ma, F. Yang, E. R. Rosario, O. J. Ubeda, W. Beech et al.

Journal of Neuroscience • 2009

DOI PMC

Y-Maze Behavioral Test

behavioralmouse3xTg-AD transgenic

Objective: Assessment of cognitive performance and spatial working memory in 3xTg-AD transgenic mice treated with dietary interventions (fish oil, curcumin, or combination)

This is a Y-Maze Behavioral Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 3 materials. Extracted from a 2009 paper published in Journal of Neuroscience.

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Replication Essentials

Model and subjects

mouse • 3xTg-AD transgenic • unknown • Not specified • Not specified

Study window

Estimated timing pending

Core workflow

Dietary intervention initiation • Treatment administration - 4 month protocol • Treatment administration - 1 month protocol

Primary readouts

Y-maze performance (spatial working memory and cognitive performance)
Phosphorylated JNK levels
Phosphorylated IRS-1 levels
Phosphorylated tau levels

Key equipment and reagents

Y-MazeFish oil

Before You Start

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Protocol Steps

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Dietary intervention initiation

3xTg-AD transgenic mice were placed on a high-fat diet (saturated and omega-6 fat) as baseline condition

Not specifiedNot specified

Note: This diet serves as the control/disease model condition

View evidence from paper

“Feeding 3xTg-AD transgenic mice a diet high in saturated and omega-6 fat increased active JNK and phosphorylated IRS-1 and tau”

Treatment administration - 4 month protocol

Mice on high-fat diet were treated with fish oil, curcumin, or a combination of both for 4 months

4 monthsNot specified

Note: Three treatment groups: fish oil alone, curcumin alone, or combination of both

View evidence from paper

“Treatment of the 3xTg-AD mice on high-fat diet with fish oil or curcumin or a combination of both for 4 months”

Treatment administration - 1 month protocol

Mice fed with fish oil and curcumin combination for 1 month showed more significant effects

1 monthNot specified

Note: The 1-month combination treatment showed more significant effects on Y-maze performance and biochemical markers than 4-month treatment

View evidence from paper

“Mice fed with fish oil and curcumin for 1 month had more significant effects on Y-maze, and the combination showed more significant inhibition of JNK, IRS-1, and tau phosphorylation”