Y-Maze Behavioral Test
Objective: Assessment of cognitive performance and spatial working memory in 3xTg-AD transgenic mice treated with dietary interventions (fish oil, curcumin, or combination)
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Equipment1
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Materials3
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Protocol Steps
Dietary intervention initiation
3xTg-AD transgenic mice were placed on a high-fat diet (saturated and omega-6 fat) as baseline condition
Note: This diet serves as the control/disease model condition
View evidence from paper
“Feeding 3xTg-AD transgenic mice a diet high in saturated and omega-6 fat increased active JNK and phosphorylated IRS-1 and tau”
Treatment administration - 4 month protocol
Mice on high-fat diet were treated with fish oil, curcumin, or a combination of both for 4 months
Note: Three treatment groups: fish oil alone, curcumin alone, or combination of both
View evidence from paper
“Treatment of the 3xTg-AD mice on high-fat diet with fish oil or curcumin or a combination of both for 4 months”
Treatment administration - 1 month protocol
Mice fed with fish oil and curcumin combination for 1 month showed more significant effects
Note: The 1-month combination treatment showed more significant effects on Y-maze performance and biochemical markers than 4-month treatment
View evidence from paper
“Mice fed with fish oil and curcumin for 1 month had more significant effects on Y-maze, and the combination showed more significant inhibition of JNK, IRS-1, and tau phosphorylation”
Y-Maze behavioral testing
Mice were tested in the Y-maze to assess spatial working memory and cognitive performance
Note: Testing was conducted to measure improvement in cognitive performance following dietary treatments
View evidence from paper
“This was accompanied by improvement in Y-maze performance. Mice fed with fish oil and curcumin for 1 month had more significant effects on Y-maze”