Source Paper
Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice
Han J, Xu X, Zhao Y, Xiao Y, Huang F et al.
Immunology • 2025
Y-Maze Test
Objective: To assess spatial memory and exploratory behaviour in mice
This is a Y-Maze Test protocol using Mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2025 paper published in Immunology.
Model and subjects
Mouse • B6SJL‐TgN(SOD1 G93A )1Gur/J transgenic mice • Not specified • 11, 14, 16, 18, and 20 weeks • 60
Study window
~8 minutes hands-on
Core workflow
Place mouse in starting arm • Record spontaneous alternation behavior • Calculate alternation percentage
Primary readouts
- Spontaneous alternation percentage
- Spatial memory performance
- Exploratory behaviour
Key equipment and reagents
Verified items
0
Direct vendor links
0
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Place mouse in starting arm
Place the mouse in the starting arm of the Y-maze
View evidence from paper
“Mice were placed in the starting arm”
Record spontaneous alternation behavior
Record spontaneous alternation behaviour, which is defined as entering a different arm on each consecutive trial
View evidence from paper
“spontaneous alternation behaviour—entering a different arm on each consecutive trial—was recorded. The test duration was 8 min”
Calculate alternation percentage
Calculate the alternation percentage as an indicator of cognitive performance
View evidence from paper
“A higher alternation percentage indicated better cognitive performance”