Source Paper
C16-siRNAs in Focus: Development of ALN-APP, a Promising RNAi-Based Therapeutic for Alzheimer’s Disease
Titze-de-Almeida R, Oliveira Gomes GD, dos Santos TC, Titze-de-Almeida SS
Pharmaceuticals (Basel) • 2025
Elevated Plus Maze
Objective: Behavioral assessment using elevated plus maze to evaluate anxiety-related behaviors and pharmacological interventions
This is a Elevated Plus Maze protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Pharmaceuticals (Basel).
Model and subjects
mouse • 5xFAD • not specified • 8 and 12 months • not specified • not specified
Study window
Estimated timing pending
Core workflow
Treatment Administration • Behavioral Testing • Data Collection
Primary readouts
- Time spent in open arms
- Number of entries into open arms
- Anxiety-related behavior assessment
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Treatment Administration
Administer C16 ALN-APP siRNAs via intracerebroventricular route at specified doses
Note: Early intervention protocol with 300 µg dose administered at 3 and 6 months; late intervention at 8 months
View evidence from paper
“300 µg administered via ICV at 3 and 6 months, referred to as robust intervention”
Behavioral Testing
Assess animals in elevated plus maze to measure anxiety-related behaviors
Note: Testing conducted at 8 and 12 months of age
View evidence from paper
“The authors further investigated the potential impact of transient, moderate, or robust treatments on behavior utilizing the elevated plus maze”
Data Collection
Record time spent in open arms and number of entries into open arms
Note: Animals typically exhibit fewer entries and less time in open arms compared to closed arms
View evidence from paper
“animals typically exhibit fewer entries into the open arms, which represent a more aversive environment, and spend less time there”