Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Elevated Plus Maze
Objective: To assess anxiety-like behavior using an elevated plus maze
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
Estimated: $1,890.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Timing
Conduct elevated plus maze testing one week after conclusion of sleep disruption
View evidence from paper
“A week after the conclusion of the SD, anxiety‐like behavior was assessed using an elevated plus maze”
Habituation
Habituate mice to the testing room for a minimum of 30 minutes prior to experimentation
View evidence from paper
“Mice were habituated to the testing room for a minimum of 30 min prior to experimentation”
Maze placement
Place the mouse in the center of the maze
View evidence from paper
“The mouse was then placed in the center of the maze”
Free exploration
Allow mouse to freely explore the maze for 10 minutes
View evidence from paper
“allowed to freely explore for 10 min”
Video recording
Record the entire session with overhead cameras
View evidence from paper
“recordings were taken with overhead cameras”
Video analysis
Analyze recorded videos using EthoVision XT software to determine time spent in different areas
View evidence from paper
“Recorded videos were analyzed using EthoVision XT software to determine the time spent on the open arms”
