Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Elevated Plus Maze
Objective: To assess anxiety-like behavior using an elevated plus maze
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Protocol Steps
Timing
Conduct elevated plus maze testing one week after conclusion of sleep disruption
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“A week after the conclusion of the SD, anxiety‐like behavior was assessed using an elevated plus maze”
Habituation
Habituate mice to the testing room for a minimum of 30 minutes prior to experimentation
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“Mice were habituated to the testing room for a minimum of 30 min prior to experimentation”
Maze placement
Place the mouse in the center of the maze
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“The mouse was then placed in the center of the maze”
Free exploration
Allow mouse to freely explore the maze for 10 minutes
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“allowed to freely explore for 10 min”
Video recording
Record the entire session with overhead cameras
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“recordings were taken with overhead cameras”
Video analysis
Analyze recorded videos using EthoVision XT software to determine time spent in different areas
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“Recorded videos were analyzed using EthoVision XT software to determine the time spent on the open arms”