Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Elevated Plus Maze
Objective: To assess anxiety-like behavior using an elevated plus maze
This is a Elevated Plus Maze protocol using mice as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Alzheimers Dement.
Model and subjects
mice • transgenic APP NL‐G‐F/NL‐G‐F knock‐in mice • male and female • 6 months • 32
Study window
~40 minutes hands-on
Core workflow
Timing • Habituation • Maze placement
Primary readouts
- time spent on the open arms with no walls (entire mouse body)
- time spent in the closed arms (walls)
- time spent in center of the maze
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Timing
Conduct elevated plus maze testing one week after conclusion of sleep disruption
View evidence from paper
“A week after the conclusion of the SD, anxiety‐like behavior was assessed using an elevated plus maze”
Habituation
Habituate mice to the testing room for a minimum of 30 minutes prior to experimentation
View evidence from paper
“Mice were habituated to the testing room for a minimum of 30 min prior to experimentation”
Maze placement
Place the mouse in the center of the maze
View evidence from paper
“The mouse was then placed in the center of the maze”
Free exploration
Allow mouse to freely explore the maze for 10 minutes
View evidence from paper
“allowed to freely explore for 10 min”
Video recording
Record the entire session with overhead cameras
View evidence from paper
“recordings were taken with overhead cameras”
Video analysis
Analyze recorded videos using EthoVision XT software to determine time spent in different areas
View evidence from paper
“Recorded videos were analyzed using EthoVision XT software to determine the time spent on the open arms”