Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Forced Swim Test
behavioralmiceC57BL/6-Ptpn5_KO and C57BL/6
Objective: To test for depressive-like behavior in mice
Protocol Steps
1
Setup reservoir
Fill cylindrical glass reservoir (height 30 cm, diameter 15 cm) half full with water at 25°C and illuminate from beneath
View evidence from paper
“clear, cylindrical glass reservoir ( h = 30 cm, d = 15 cm) half filled with water ( T = 25°С) and illuminated from beneath”
2
Place mouse in water
Carefully place mouse into the water for total duration of 6 minutes
View evidence from paper
“A mouse was carefully placed into the water for 6 min”
3
Exclude adaptation period
Do not analyze the first 2 minutes as this is the adaptive period
View evidence from paper
“The first 2 min of the test are adaptive and were not analyzed”
4
Record immobility behavior
Evaluate total immobility time during the latter 4 minutes by experienced rater
View evidence from paper
“For the latter 4 min, the total immobility time was evaluated by an experienced rater”