Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Forced Swim Test
Objective: To test for depressive-like behavior in mice
This is a Forced Swim Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 • male • 2-month-old adult • not specified
Study window
~12 minutes hands-on
Core workflow
Setup reservoir • Place mouse in water • Exclude adaptation period
Primary readouts
- total immobility time during final 4 minutes
- depressive-like behavior assessment
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Setup reservoir
Fill cylindrical glass reservoir (height 30 cm, diameter 15 cm) half full with water at 25°C and illuminate from beneath
View evidence from paper
“clear, cylindrical glass reservoir ( h = 30 cm, d = 15 cm) half filled with water ( T = 25°С) and illuminated from beneath”
Place mouse in water
Carefully place mouse into the water for total duration of 6 minutes
View evidence from paper
“A mouse was carefully placed into the water for 6 min”
Exclude adaptation period
Do not analyze the first 2 minutes as this is the adaptive period
View evidence from paper
“The first 2 min of the test are adaptive and were not analyzed”
Record immobility behavior
Evaluate total immobility time during the latter 4 minutes by experienced rater
View evidence from paper
“For the latter 4 min, the total immobility time was evaluated by an experienced rater”