Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Forced Swim Test
Objective: To assess animal mobility and swimming behavior as a measure of behavioral despair or antidepressant-like effects
This is a Forced Swim Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Biomolecules.
Model and subjects
mice • C57BL/6 • male • 8-10 weeks • 25
Study window
~4 minutes hands-on
Core workflow
Pre-test preparation • Place mouse in cylinder • Habituation period
Primary readouts
- Rate of change in animal's silhouette (mobility measure defined as number of pixels that changed between consecutive frames)
Key equipment and reagents
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Protocol Steps
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Pre-test preparation
Single-house animals two days before testing
View evidence from paper
“Two days before testing, animals were weighed and single-housed”
Place mouse in cylinder
Place each mouse in the water-filled glass cylinder
View evidence from paper
“Each mouse was placed in a transparent glass cylinder”
Habituation period
Allow 2-minute habituation period before recording
View evidence from paper
“After a 2 min habituation period”
Record mobility
Automatically record animal mobility for 4 minutes using software that measures pixel changes in the animal's silhouette
View evidence from paper
“the animal's mobility was recorded automatically for 4 min using custom EthoStudio software”