Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Forced Swim Test
behavioralmiceC57BL/6
Objective: To assess animal mobility and swimming behavior as a measure of behavioral despair or antidepressant-like effects
Protocol Steps
1
Pre-test preparation
Single-house animals two days before testing
View evidence from paper
“Two days before testing, animals were weighed and single-housed”
2
Place mouse in cylinder
Place each mouse in the water-filled glass cylinder
View evidence from paper
“Each mouse was placed in a transparent glass cylinder”
3
Habituation period
Allow 2-minute habituation period before recording
View evidence from paper
“After a 2 min habituation period”
4
Record mobility
Automatically record animal mobility for 4 minutes using software that measures pixel changes in the animal's silhouette
View evidence from paper
“the animal's mobility was recorded automatically for 4 min using custom EthoStudio software”