Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Home Cage Activity
Objective: Assess daily locomotor activity, sleep duration, and food and water consumption
This is a Home Cage Activity protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 • male • 2-month-old • not specified
Study window
~96 hours hands-on
Core workflow
Assessment period • Adaptation period • Data analysis period
Primary readouts
- Locomotor activity (distance traveled during each hour in meters)
- Sleep data (cumulative sleep duration in minutes during each hour)
- Food consumption (quantity in grams ingested during every 2 hours)
- Water consumption (quantity in milliliters ingested during every 2 hours)
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Assessment period
Assess daily locomotor activity, sleep duration, and food and water consumption for 72 hours total
View evidence from paper
“Daily locomotor activity, sleep duration, and food and water consumption were assessed for 72 h”
Adaptation period
Exclude the first 24 hours as adaptive period
View evidence from paper
“The first 24 h (1–24 h) were considered as adaptive and were not taken into account”
Data analysis period
Analyze home cage activity from hours 25-72 and average for one representative 24-hour period
View evidence from paper
“Home cage activity of hours 25–72 was analyzed and averaged for one representative 24 h”