Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Hotplate test
behavioralmiceC57BL/6 N
Objective: To evaluate nociceptive sensitivity in mice
Materials & Equipment Checklist
2 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
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Protocol Steps
1
Place mouse on heated plate
Place the mouse on a heated plate maintained at 55.0 ± 0.3 °C
View evidence from paper
“Mice were placed on a heated plate maintained at 55.0 ± 0.3 °C”
2
Record latency to first paw response
Record the time until the first paw response (hind paw licking, shaking, or trembling) occurs
View evidence from paper
“the latency to the first paw response (e.g., hind paw licking, shaking, or trembling) was recorded”
3
Apply safety cut-off
Use a maximum cut-off time of 30 seconds to prevent tissue damage
View evidence from paper
“To prevent tissue damage, a cut-off time of 30 s was applied”
4
Single test per mouse
Test each mouse only once and use the recorded latency for analysis
View evidence from paper
“Each mouse was tested once, and the latency was used for analysis”