Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Intraperitoneal Glucose Tolerance Test
Objective: To assess glucose tolerance and blood glucose response over time
This is a Intraperitoneal Glucose Tolerance Test protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
mice • C57BL/6 • male • 6-week-old • 36
Study window
~10 week study window | ~12 hours hands-on
Core workflow
Dietary conditioning • Fasting period • Glucose injection
Primary readouts
- Blood glucose levels at 0, 30, 60, and 120 minutes
- Blood glucose tolerance assessment
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Dietary conditioning
Feed mice with cafeteria diet for 10 weeks
View evidence from paper
“After a 10‐week CAF diet, the IPGTT was performed”
Fasting period
Fast mice for 12 hours before the test
View evidence from paper
“the IPGTT was performed after fasting for 12 h”
Glucose injection
Inject glucose intraperitoneally at 2 g/kg body weight
View evidence from paper
“glucose was injected into mice at 2 g/kg of body weight”
Blood glucose monitoring
Measure blood glucose at baseline and multiple time points
View evidence from paper
“Blood glucose was measured at zero time and 30, 60 and 120 min”
Assessment
Assess blood glucose tolerance based on measurements
View evidence from paper
“to assess the blood glucose tolerance”