Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Intraperitoneal Glucose Tolerance Test (IPGTT)
Objective: To assess glucose tolerance and insulin sensitivity in mice following dietary interventions
This is a Intraperitoneal Glucose Tolerance Test (IPGTT) protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
mice • C57BL/6 • male • 6-week-old • 36
Study window
~2 hour study window | ~12 hours hands-on
Core workflow
Fasting period • Baseline glucose measurement • Glucose injection
Primary readouts
- Blood glucose tolerance
- Area under the curve (AUC) for glucose response
Key equipment and reagents
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Protocol Steps
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Fasting period
Fast mice for 12 hours prior to testing
View evidence from paper
“the IPGTT was performed after fasting for 12 h”
Baseline glucose measurement
Measure blood glucose at time zero before glucose injection
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“Blood glucose was measured at zero time”
Glucose injection
Inject glucose intraperitoneally at 2 g/kg body weight
View evidence from paper
“glucose was injected into mice at 2 g/kg of body weight”
Post-injection glucose measurements
Measure blood glucose at 30, 60, and 120 minutes after injection
View evidence from paper
“Blood glucose was measured at zero time and 30, 60 and 120 min”