Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Light/dark transition test
behavioralmiceC57BL/6 N
Objective: To evaluate anxiety-like behavior using the light/dark transition test
Materials & Equipment Checklist
7 items
Protocol Steps
1
Test timing
Conduct test during light phase between 10:00 and 17:00
View evidence from paper
“All behavioral tests were conducted in a dedicated testing room during the light phase of the light/dark cycle, between 10:00 and 17:00”
2
Initial placement
Place mouse in the dark chamber at start of test
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“At the start of the test, each mouse was placed in the dark chamber”
3
Free exploration period
Allow mouse to freely explore both compartments for 6 minutes
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“allowed to freely explore both compartments for 6 min”
4
Data collection
Record behavioral parameters using ANY-maze software
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“Behavioral data were collected and analyzed using ANY-maze software”