Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Light/dark transition test
Objective: To evaluate anxiety-like behavior using the light/dark transition test
This is a Light/dark transition test protocol using mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 5 materials. Extracted from a 2026 paper published in IBRO Neurosci Rep.
Model and subjects
mice • C57BL/6 N • male • Eight-week-old • 20
Study window
~6 minutes hands-on
Core workflow
Test timing • Initial placement • Free exploration period
Primary readouts
- total distance traveled (m)
- number of entries into the light chamber
- time spent in the light chamber (s)
- latency to the first entry into the light chamber (s)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Test timing
Conduct test during light phase between 10:00 and 17:00
View evidence from paper
“All behavioral tests were conducted in a dedicated testing room during the light phase of the light/dark cycle, between 10:00 and 17:00”
Initial placement
Place mouse in the dark chamber at start of test
View evidence from paper
“At the start of the test, each mouse was placed in the dark chamber”
Free exploration period
Allow mouse to freely explore both compartments for 6 minutes
View evidence from paper
“allowed to freely explore both compartments for 6 min”
Data collection
Record behavioral parameters using ANY-maze software
View evidence from paper
“Behavioral data were collected and analyzed using ANY-maze software”