Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Modified SHIRPA Screen
Objective: Assessment of neurological phenotypes including lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape
This is a Modified SHIRPA Screen protocol using mice as the model organism. The procedure involves 4 procedural steps. Extracted from a 2025 paper published in Mol Ther Nucleic Acids.
Model and subjects
mice • Not specified • Not specified • 4 weeks • Not specified
Study window
~4 week study window
Core workflow
Home cage observation • Phenotype assessment • Scoring system
Primary readouts
- Lethargy level
- Gait issues
- Tremors
- Pelvic and tail elevation
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Home cage observation
Observe mice inside their home cage to assess multiple neurological phenotypes
View evidence from paper
“In our modified SHIRPA we observed mice inside of their home cage”
Phenotype assessment
Assess level of lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape using individual scales
View evidence from paper
“to assess their level of lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape”
Scoring system
Use individual scale for each phenotype with higher scores indicating worsening phenotype
View evidence from paper
“Each phenotype that was assessed had an individual scale that was used to measure the behavior”
Age of assessment
Assess all mice at 4 weeks of age
View evidence from paper
“All mice were assessed at 4 weeks of age”