Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Modified SHIRPA Screen
behavioralmiceNot specified
Objective: Assessment of neurological phenotypes including lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape
Protocol Steps
1
Home cage observation
Observe mice inside their home cage to assess multiple neurological phenotypes
View evidence from paper
“In our modified SHIRPA we observed mice inside of their home cage”
2
Phenotype assessment
Assess level of lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape using individual scales
View evidence from paper
“to assess their level of lethargy, gait issues, tremors, pelvic and tail elevation, hindlimb and forelimb grasping, piloerection, and touch escape”
3
Scoring system
Use individual scale for each phenotype with higher scores indicating worsening phenotype
View evidence from paper
“Each phenotype that was assessed had an individual scale that was used to measure the behavior”
4
Age of assessment
Assess all mice at 4 weeks of age
View evidence from paper
“All mice were assessed at 4 weeks of age”