Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Morris Water Maze
Objective: Spatial memory behavioral test
This is a Morris Water Maze protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2025 paper published in Cell Death Dis.
Model and subjects
mice • C57BL/6J and SAMP8 • Male and female • 3 and 20-month-old C57BL/6J mice, and 2 and 10-month-old SAMP8 mice • 40
Study window
~1.4 week study window
Core workflow
Training setup • Training schedule • Trial parameters
Primary readouts
- Latency time (in seconds) required for the animal to reach the platform
- Time spent in each quadrant (in seconds)
- Spatial acuity score calculated from time in quadrant and platform area
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Training setup
Train mice in circular pool with submerged platform for spatial learning
View evidence from paper
“Each animal was trained to locate the platform”
Training schedule
Conduct three trials per day for 10 days excluding weekends
View evidence from paper
“The test was performed with three trials per day for 10 days except for weekends”
Trial parameters
Set maximum trial duration of 60 seconds with 2 seconds on platform at end
View evidence from paper
“maximum trial duration of 60 s and 2 s on the platform at the end of the trials”
Probe test
Remove platform on day 11 for probe test
View evidence from paper
“probe test was carried out on day 11, when the platform was removed”
Post-trial handling
Gently remove mouse from maze and return to cage after testing
View evidence from paper
“After testing, the mouse was gently removed from the maze and returned to its cage”