Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Morris Water Maze
behavioralmiceC57BL/6J and SAMP8
Objective: Spatial memory behavioral test
Protocol Steps
1
Training setup
Train mice in circular pool with submerged platform for spatial learning
View evidence from paper
“Each animal was trained to locate the platform”
2
Training schedule
Conduct three trials per day for 10 days excluding weekends
View evidence from paper
“The test was performed with three trials per day for 10 days except for weekends”
3
Trial parameters
Set maximum trial duration of 60 seconds with 2 seconds on platform at end
View evidence from paper
“maximum trial duration of 60 s and 2 s on the platform at the end of the trials”
4
Probe test
Remove platform on day 11 for probe test
View evidence from paper
“probe test was carried out on day 11, when the platform was removed”
5
Post-trial handling
Gently remove mouse from maze and return to cage after testing
View evidence from paper
“After testing, the mouse was gently removed from the maze and returned to its cage”