Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Morris Water Maze
Objective: To test spatial learning and memory
This is a Morris Water Maze protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 • male • 2-month-old • not specified
Study window
Estimated timing pending
Core workflow
Acquisition phase training • Daily testing parameters • Retention test
Primary readouts
- Latency time (s): the time for the mouse to find the platform location
- Path (m) traveled by the animal from the moment it was placed into the water till it found the platform
- Cumulative distance (m) between the geometric center of the mouse and the platform
- Time spent in target sector during retention test
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Acquisition phase training
Train mice for four consecutive days to find the platform
View evidence from paper
“During the acquisition phase for four consecutive days, mice were trained to find the platform”
Daily testing parameters
Record latency time, path traveled, and cumulative distance for three attempts per day
View evidence from paper
“For each day, latency, path, and distance were calculated as the average of the three attempts”
Retention test
On day 5, remove platform and place mouse at center of pool
View evidence from paper
“On day 5, the retention test took place. The platform was removed and a mouse was placed at the center”
Data analysis
Average results over three attempts and assess if time in target sector exceeds 25%
View evidence from paper
“Results were averaged over the three attempts. A statistically significant excess of the 25% of time spent in the target sector”