Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Morris Water Maze
behavioralmiceC57BL/6-Ptpn5_KO and C57BL/6
Objective: To test spatial learning and memory
Materials & Equipment Checklist
3 items
Protocol Steps
1
Acquisition phase training
Train mice for four consecutive days to find the platform
View evidence from paper
“During the acquisition phase for four consecutive days, mice were trained to find the platform”
2
Daily testing parameters
Record latency time, path traveled, and cumulative distance for three attempts per day
View evidence from paper
“For each day, latency, path, and distance were calculated as the average of the three attempts”
3
Retention test
On day 5, remove platform and place mouse at center of pool
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“On day 5, the retention test took place. The platform was removed and a mouse was placed at the center”
4
Data analysis
Average results over three attempts and assess if time in target sector exceeds 25%
View evidence from paper
“Results were averaged over the three attempts. A statistically significant excess of the 25% of time spent in the target sector”