Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Novel Object Localization
behavioralmiceC57BL/6J and SAMP8
Objective: To assess spatial localization memory by measuring the animal's ability to recognize when a familiar object has been moved to a new location
Protocol Steps
1
Habituation
Place animals in the test box without objects for habituation
View evidence from paper
“The animals were habituated in the box for 5 minutes twice on the first day without any object”
2
Novel Object Recognition test
Perform NOR test with two identical objects for 10 minutes, then after 2 hours expose animal to one old object and one new object
View evidence from paper
“for the NOR test, each animal was placed in the box containing two identical objects (old objects) for 10 min. After 2 h, the animal was exposed to one of the old objects and a new object”
3
Novel Object Localization test timing
Perform NOL test 2 hours after the NOR test
View evidence from paper
“NOL test was performed 2 h after NOR test”
4
Object location change
Expose animals to the same old object and change the location of the previous novel object
View evidence from paper
“the animals were exposed to the same old object, and the previous novel object's location was changed”
5
Cleaning between animals
Clean the box chamber with ethanol between each animal
View evidence from paper
“After each test, the box chamber was cleaned with ethanol before a different mouse was tested”