Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Novel Object Recognition Test
Objective: To assess recognition memory and cognitive function in mice
This is a Novel Object Recognition Test protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
mice • C57BL/6 • male • 6-week-old • 36
Study window
~5 minutes hands-on
Core workflow
Day 1 - Training session • Day 2 - Test session • Duration of test
Primary readouts
- Exploration time of objects
- Recognition memory assessment
Key equipment and reagents
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Protocol Steps
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Day 1 - Training session
Place mice individually in arena with two identical objects and allow exploration for 5 minutes
View evidence from paper
“On day one (training session), mice were placed in the arena with two identical objects and allowed to explore for 5 min”
Day 2 - Test session
Replace one identical object with a novel object and record exploration time
View evidence from paper
“On day two (test session), one object was replaced with a novel object, and exploration time was recorded”
Duration of test
The NOR test is conducted over two consecutive days
View evidence from paper
“The NOR behavioural test was conducted for two consecutive days”