Source Paper
The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice
He D, He R, Duan W, Li G, Kang Q
Future Sci OA • 2026
Novel Object Recognition Test
Objective: To assess recognition memory and cognitive function by measuring the ability to distinguish between familiar and novel objects
Gather these items before starting the experiment. Check off items as you prepare.
Equipment3
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Protocol Steps
Pre-habituation
Each mouse was individually placed into the behavioral apparatus for habituation to the environment
View evidence from paper
“One day before the test, each mouse was individually placed into the behavioral apparatus for 1 hour to habituate”
Habituation phase
Each test mouse was placed in the center of the apparatus and allowed to move freely to fully explore the entire apparatus
View evidence from paper
“each test mouse was placed in the center of the apparatus and allowed to move freely to fully explore the entire apparatus for 10 minutes”
Training phase
Each test mouse was individually placed in the center of the apparatus with two identical blue cylinders placed at the two top corners and allowed to explore freely
View evidence from paper
“Each test mouse was individually placed in the center of the apparatus and allowed to move freely for 10 minutes to fully explore, with two identical blue cylinders (object 1) placed at the two top corners”
Recognition phase
Each test mouse was placed in the center of the apparatus with one blue cylinder and one green cone placed at the top corners and allowed to explore freely
View evidence from paper
“Each test mouse was placed in the center of the apparatus and allowed to move freely to fully explore for 10 minutes, with one blue cylinder (object 1) and one green cone (object 2) placed at the top corners”
Video analysis
Time spent by mice interacting with each object was analyzed using video tracking software, with interaction defined as nose tip distance ≤ 3 cm from object
View evidence from paper
“the time spent by the mice interacting with each object (object 1) or the new object (object 2) (with the distance from the nose tip to the object ≤ 3 cm) was analyzed using Ethovision XT software”