Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Novel Object Recognition Test
Objective: To assess recognition memory by measuring differential exploration time of novel versus familiar objects
This is a Novel Object Recognition Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 3 equipment items, 2 materials. Extracted from a 2026 paper published in Biomolecules.
Model and subjects
mice • C57BL/6 • male • 8-10 weeks • 40
Study window
Estimated timing pending
Core workflow
Habituation • Familiarization • Testing phase
Primary readouts
- Object exploration time (defined as approaching, sniffing, or making physical contact with snout or forepaws)
- Ratio of time spent exploring novel object to total time spent exploring both objects
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Habituation
Place each mouse in the empty arena for habituation
View evidence from paper
“On the first day, each mouse was habituated to the empty arena for 10 min”
Familiarization
Present two identical objects symmetrically placed in the arena for exploration
View evidence from paper
“On the second day, two identical, previously unfamiliar objects were placed symmetrically relative to the center of the arena, and the mouse was allowed to explore them for 10 min”
Testing phase
Replace one familiar object with a novel object and record exploration behavior
View evidence from paper
“On the third day, one of the familiar objects was replaced with a novel object differing in shape, material, or color, and the exploration time of both objects was recorded for 10 min”
Arena cleaning
Clean arena between test sessions
View evidence from paper
“The arena was cleaned after each test session”