Experiments/Novel Object Recognition Test

Source Paper

Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation

Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.

Alzheimers Dement • 2026

PMC

Novel Object Recognition Test

behavioralmicetransgenic APP NL‐G‐F/NL‐G‐F knock‐in mice

Objective: To examine recognition memory using a Novel Object Recognition test

This is a Novel Object Recognition Test protocol using mice as the model organism. The procedure involves 8 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in Alzheimers Dement.

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Replication Essentials

Model and subjects

mice • transgenic APP NL‐G‐F/NL‐G‐F knock‐in mice • both male and female • 6 months • 32

Study window

~1 hours hands-on

Core workflow

Test timing • Room habituation • Arena habituation

Primary readouts

Recognition memory performance
Object exploration behavior

Key equipment and reagents

PhenoTypersEthoVision XT software

Before You Start

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Protocol Steps

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Test timing

Conduct the Novel Object Recognition test four days after conclusion of sleep disruption

View evidence from paper

“Four days after the conclusion of the SD, mice were subjected to a Novel Object Recognition (NOR) test”

Room habituation

Habituate mice to the testing room for 30 minutes prior to experimentation

View evidence from paper

“Mice were habituated to the testing room for 30 min prior to experimentation”

Arena habituation

Place mice individually in empty PhenoTypers to habituate to the arena for 10 minutes

View evidence from paper

“mice were individually placed in empty PhenoTypers to habituate to the arena for 10 min”

Break period

Provide a 1-hour break after arena habituation

View evidence from paper

“After a 1‐h break, the familiarization phase was conducted”

Familiarization phase

Place mice in the same PhenoTyper containing two identical objects (either LEGO towers or T25 flasks) and allow free exploration for 10 minutes

View evidence from paper

“mice were placed in the same PhenoTyper containing either two LEGO towers or two T25 cell culture flasks filled with bedding and allowed to freely explore the objects for 10 min”

Second break period

Provide another 1-hour break after the familiarization phase

View evidence from paper

“After another 1‐h break, the NOR test was conducted”