Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Open Field
Objective: Assess locomotor activity and anxiety-like behavior in an open field arena
This is a Open Field protocol using mice as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 2 materials. Extracted from a 2025 paper published in Mol Ther Nucleic Acids.
Model and subjects
mice • not specified • Male and female mice were used in all experiments • not specified for open field cohort • not specified
Study window
~12 minutes hands-on
Core workflow
Place mouse in arena center • Record video session • Analyze behavior quantitatively
Primary readouts
- total distance traveled
- freezing to motion ratio
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Place mouse in arena center
Place the mouse in the center of the open field arena
View evidence from paper
“Mice were placed in the center of a 41 × 41 cm closed box”
Record video session
Record mouse behavior for 12 minutes using mounted GoPro camera
View evidence from paper
“A GoPro camera, mounted in the lid, recorded a 12-min video of each individual mouse”
Analyze behavior quantitatively
Process video data using ezTrack software to measure locomotor activity and freezing behavior
View evidence from paper
“Quantitative analysis was performed using Cai Lab's ezTrack script to measure total distance traveled and the freezing to motion ratio”