Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Open Field Test
Objective: To assess locomotor activity and anxiety-like behaviours
This is a Open Field Test protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
mice • C57BL/6 • male • 6-week-old • 36
Study window
~5 minutes hands-on
Core workflow
Individual placement in arena • Record movement • Analyze spatial behavior
Primary readouts
- Time spent in centre versus periphery
- Distance travelled in centre versus periphery
- Locomotor activity
- Anxiety-like behaviours
Key equipment and reagents
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Protocol Steps
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Individual placement in arena
Place mice individually in the square arena
View evidence from paper
“Mice were placed individually in a square arena (40 cm × 40 cm)”
Record movement
Record mouse movement for 5 minutes using video tracking system
View evidence from paper
“their movement was recorded for 5 min using a video tracking system”
Analyze spatial behavior
Analyze time spent and distance travelled in center versus periphery areas
View evidence from paper
“The time spent and distance travelled in the centre versus periphery were analysed”