Source Paper
Changes in gut, microbiome, and cognition after doxorubicin, cyclophosphamide, and paclitaxel chemotherapy treatment
Cronin B, Kandel S, McElroy T, Syed S, Swinton C et al.
Sci Rep • 2026
Open Field Test
Objective: To assess the unconditioned anxiety of mice
This is a Open Field Test protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Sci Rep.
Model and subjects
mice • C57/BL6J • female • Twelve-week-old • not specified
Study window
~10 minutes hands-on
Core workflow
Prepare arena • Place mouse in arena • Allow free exploration
Primary readouts
- Total distance traveled (cm)
- Velocity (cm/s)
- Time spent in center zone (20 cm x 20 cm)
- Number of feces
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Prepare arena
Set up square Plexiglass arena with gray floor and cover walls with white posterboard to prevent mouse from seeing room surroundings
View evidence from paper
“The walls of the arena were covered with white posterboard to prevent the mouse from seeing the room's surroundings”
Place mouse in arena
Place the mouse in the middle of the arena
View evidence from paper
“The mice were placed in the middle of a square Plexiglass arena”
Allow free exploration
Allow the mouse to roam freely for 10 minutes
View evidence from paper
“The mouse was allowed to roam freely for 10 min”
Count feces
At the end of the trial, count the number of feces
View evidence from paper
“At the end of the trial, the number of feces were counted”
Clean arena
Clean the arena with 20% ethanol between animals to minimize olfactory signals
View evidence from paper
“The arena was cleaned with 20% ethanol between animals to minimize olfactory signals between trials”