Source Paper
Changes in gut, microbiome, and cognition after doxorubicin, cyclophosphamide, and paclitaxel chemotherapy treatment
Cronin B, Kandel S, McElroy T, Syed S, Swinton C et al.
Sci Rep • 2026
Open Field Test
behavioralmiceC57/BL6J
Objective: To assess the unconditioned anxiety of mice
Protocol Steps
1
Prepare arena
Set up square Plexiglass arena with gray floor and cover walls with white posterboard to prevent mouse from seeing room surroundings
View evidence from paper
“The walls of the arena were covered with white posterboard to prevent the mouse from seeing the room's surroundings”
2
Place mouse in arena
Place the mouse in the middle of the arena
View evidence from paper
“The mice were placed in the middle of a square Plexiglass arena”
3
Allow free exploration
Allow the mouse to roam freely for 10 minutes
View evidence from paper
“The mouse was allowed to roam freely for 10 min”
4
Count feces
At the end of the trial, count the number of feces
View evidence from paper
“At the end of the trial, the number of feces were counted”
5
Clean arena
Clean the arena with 20% ethanol between animals to minimize olfactory signals
View evidence from paper
“The arena was cleaned with 20% ethanol between animals to minimize olfactory signals between trials”